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芪参益气滴丸对气虚血瘀证心肌梗死大鼠的心肌保护机制

发表时间:2012-12-15  浏览量:1073  下载量:463
全部作者: 许颖智,张军平,杨萃,庞树朝,王小玲,林杨
作者单位: 天津中医药大学第一附属医院老年病科;天津中医药大学研究生院
摘 要: 目的:观察芪参益气(Qishenyiqi,QSYQ)滴丸的心肌保护作用及其对糖原合成酶激酶(glycogen synthase kinase-3β,GSK-3β)通路的影响机制。方法:雄性SD(sprague dawley)大鼠50只,随机分为正常组、模型对照组、实验模型组、消心痛(isosorbide dinitrate,ISD)组及QSYQ组,每组10只。建立心梗模型和气虚血瘀证心梗模型,分别用ISD和QSYQ滴丸干预,观察心脏射血分数(ejection fraction,EF)、心肌梗死情况和心肌组织的GSK-3β,Toll样受体4(Toll like receptor 4,TRL4)、核因子-κB(nuclear factor-κB,NF-κB)及β-catenin的蛋白和基因表达情况。结果:取后心肌组织氯化硝基四氮唑蓝(nitroblue tetrazol ium,NBT)染色后,正常组心肌组织显示紫黑色;各模型组的心肌组织可见有大片的灰白色梗死区,各给药组的心肌组织可见有较小的灰白色梗死区。各模型组EF明显低于正常组,ISD组、QSYQ组均能改善心功能,有统计学意义(P<0.01)。免疫组化结果显示,正常组GSK-3β和TRL4蛋白阳性表达细胞百分比较低,低于各模型组(P<0.01);ISD和QSYQ滴丸能够降低心肌组织GSK-3β和TRL4蛋白的阳性表达(P<0.01)。Real-time PCR (RT-PCR)结果显示,正常组β-catenin基因相对表达量最高,NF-κB基因相对表达量最低,各模型组β-catenin基因表达低于正常组,NF-κB基因表达高于正常组(P<0.01);ISD和QSYQ滴丸能够促进β-catenin基因表达,抑制NF-κB基因表达(P<0.01)。结论:QSYQ滴丸可能通过调节TRL4/NF-κB,GSK-3β/β-catenin通路抑制心肌细胞凋亡,从而实现对心肌的保护作用。
关 键 词: 中西医结合医学;芪参益气滴丸;气虚血瘀证心肌梗死;GSK-3β通路
Title: Protection of Qishengyiqi dripping pills on rats with myocardial infarction of Qi deficiency and blood stasis
Author: XU Yingzhi, ZHANG Junping, YANG Cui, PANG Shuchao, WANG Xiaoling, LIN Yang
Organization: Department of Geratology, First Teaching Hospital of Tianjin University of Traditional Chinese Medicine; Graduate School of Tianjin University of Traditional Chinese Medicine
Abstract: Objective: To observe the protection of Qishenyiqi (QSYQ) dripping pills and its influence mechanism on glycogen synthase kinase-3β (GSK-3β) pathway. Methods: 50 male SD (sprague dawley) rats were randomly divided into normal group, model control group, experimental model group, isosorbide dinitrate (ISD) group and QSYQ group, each group included 10 rats. The myocardial infarction model and the deficiency of Qi and blood stasis myocardial infarction model were established respectively. With ISD and QSYQ dripping pill intervention, cardiac ejection fraction (EF), myocardial infarction and protein and gene expression of GSK-3β, Toll like receptor 4 (TRL4), nuclear factor-κB (NF-κB), β-catenin in myocardial tissue were observed. Results: After nitroblue tetrazol ium (NBT) staining, myocardial tissue in the normal group was stained purple; Large gray infracted regions and smaller gray infarction areas in myocardial tissue were found in model groups and QSYQ group respectively. The EF in the model groups was significantly lower than that in the normal group; The cardiac function was significantly improved in ISD and QSYQ group (P<0.01). Immunohistochemical results showed that the number of cells with positive expression of GSK-3β and TRL4 proteins decreased in the normal group, which was lower than that in model group (P<0.01); ISD and QSYQ dripping pills could decrease the expression of GSK-3β and TRL4 protein (P<0.01). Real-time PCR (RT-PCR) results showed that the expression level of β-catenin gene was the highest while the expression level of NF-κB gene was the lowest in the normal group. The expression level of β-catenin gene in the model group was lower than that in normal group, while the xepression level of NF-κB gene in the model group was higher than that in normal group (P<0.01); Both ISD and QSYQ dripping pills could promote the expression of β-catenin gene, while inhibit the expression of NF-κB gene (P<0.01). Conclusion: QSYQ dripping pills could inhibit the death of myocardial cells through regulating TRL4/NF-κB, GSK-3β/β-catenin pathway, thus achieved the protection effect on myocardial.
Key words: combined therapy of Chinese and Western; Qishenyiqi pills; the deficiency of Qi and blood stasis myocardial infarction model; glycogen synthase kinase-3β pathway
发表期数: 2012年12月第23期
引用格式: 许颖智,张军平,杨萃,等. 芪参益气滴丸对气虚血瘀证心肌梗死大鼠的心肌保护机制[J]. 中国科技论文在线精品论文,2012,5(23):2290-2295.
 
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