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SiO2包裹金纳米棒制备双模式光学细胞成像探针

发表时间:2013-02-15  浏览量:2177  下载量:721
全部作者: 宗慎飞,王著元,陈辉,王春雷,徐淑宏,崔一平
作者单位: 东南大学电子科学与工程学院先进光子学中心
摘 要: 提出一种新型的双模式光学细胞成像探针。该探针能提供荧光和表面增强拉曼散射(surface enhanced Raman scattering, SERS)信号对细胞进行成像。该探针具有核壳型结构,以金纳米棒(gold nanorods, GNRs)为核,其上标记拉曼分子产生SERS信号。随后在GNRs表面包裹一层SiO2并吸附量子点(quantum dots, QDs)以产生荧光信号。最后,为使该探针具有较好的生物兼容性,探针最外层包裹上聚合物电解质(polyelectrolytes, PEs)。将该探针置入活细胞中后,通过选用不同的激发光波长,实现了细胞的荧光及SERS双模式成像。该探针在癌症诊断及肿瘤靶向等领域具有很大的应用前景。
关 键 词: 光学工程;表面增强拉曼散射;荧光;金纳米棒;量子点;细胞成像
Title: A dual-mode cell imaging probe based on silica coated gold nanorods
Author: ZONG Shenfei, WANG Zhuyuan, CHEN Hui, WANG Chunlei, XU Shuhong, CUI Yiping
Organization: Advanced Photonics Center, School of Electronic Science and Engineering, Southeast University
Abstract: In this paper, a dual-mode surface enhanced Raman scattering (SERS) and fluorescence probe was proposed for cell imaging. In the fabrication process of the probe, SERS reporter molecules were first attached to the surfaces of the gold nanorods (GNRs) to generate SERS signals. Then, the surface of GNRs was coated with SiO2. Next, quantum dots (QDs) were adsorbed onto the SiO2 layer to generate fluorescence signals. Finally, the outmost surface of the nanoparticles were wrapped by polyelectrolytes (PEs) to improve the biocompatibility of the probes. After putting the probes into living cells, the dual-mode cell imaging of both SERS and fluorescence could be obtained through selecting different exciting wavelengths. This probe has a great potential application in cancer diagnosis and tumor targeting.
Key words: optical engineering; surface enhanced Raman scattering; fluorescence; gold nanorods; quantum dots; cell imaging
发表期数: 2013年2月第3期
引用格式: 宗慎飞,王著元,陈辉,等. SiO2包裹金纳米棒制备双模式光学细胞成像探针[J]. 中国科技论文在线精品论文,2013,6(3):203-209.
 
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