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应用荧光定量PCR比较Ct值法测定基因相对表达量

发表时间:2013-03-15  浏览量:16602  下载量:8183
全部作者: 魏洁书,杨锦芬
作者单位: 广州中医药大学中药资源科学与工程研究中心,岭南中药资源教育部重点实验室
摘 要: 荧光定量PCR比较Ct值法是利用荧光定量PCR测定基因相对表达量常用的方法。这里通过实验操作的具体说明,介绍了比较Ct值法的原理和特点,并对比较Ct值法测定基因相对表达量的关键步骤和技术细节,如引物设计、cDNA模板的制备、内参基因的筛选、定量PCR体系的优化及实验数据的分析等进行综述。同时对比较Ct值法的应用前景进行了总结和展望,为比较Ct值法在基因相对表达量测定中的应用提供了详细的参考资料。
关 键 词: 分子生物学;基因表达;综述;荧光定量PCR;比较Ct值法
Title: Application of real-time fluorescent quantitative PCR based on the Ct value comparison method to determine the relative gene expression
Author: WEI Jieshu, YANG Jinfen
Organization: Research Center of Chinese Herbal Resource Science and Engineering, Key Laboratory of Chinese Medicinal Resource from Lingnan, Guangzhou University of Chinese Medicine, Ministry of Education
Abstract: Ct value comparison method is the commonly used method of fluorescent quantitative PCR determination for relative gene expression. In this paper, through the description of concrete operations, the process and characteristics of Ct value comparison method was described. Furthermore, this paper introduced the key steps and technical details of the Ct value comparison method for the determination of the relative gene expression such as primer design, cDNA templet preparason, reference gene screening, optimization of quantitative PCR system and analysis of experimental data. In addition, the application of Ct value comparison method was summarized and prospected, which provided detailed references for the application of Ct value comparison method in determining the relative gene expression.
Key words: molecular biology; gene expression; review; fluorescent quantitative PCR; Ct value comparison method
发表期数: 2013年3月第5期
引用格式: 魏洁书,杨锦芬. 应用荧光定量PCR比较Ct值法测定基因相对表达量[J]. 中国科技论文在线精品论文,2013,6(5):390-395.
 
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