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家蚕蛹中30 ku蛋白群与壳聚糖轭合物对提高蛋白质口服吸收利用度的实验研究

发表时间:2013-03-15  浏览量:1060  下载量:382
全部作者: 姜啸风,于飞,方腾飞,廖必锋,杨炳飞,张耀洲
作者单位: 浙江大学生命科学学院;天津市国际生物医药联合研究院
摘 要: 为探索提高目的蛋白的口服吸收利用度,研究建立了蚕蛹粉中30 ku蛋白群以及壳聚糖的制备工艺标准。利用蚕蛹粉30 ku蛋白群与壳聚糖构成的轭合物包裹重组人粒细胞—巨噬细胞集落刺激因子(recombinant human granulocyte macrophage colony stimulating factor,rhGM-CSF),灌胃促进小鼠对rhGM-CSF的吸收;同时,利用此轭合物与增强型绿色荧光蛋白(enhanced green fluorescence protein,EGFP)混合,以提高EGFP在人工消化液中的稳定性。ELISA检测表明:用此轭合物包裹rhGM-CSF后给小鼠灌胃,小鼠血清中的rhGM-CSF含量比单独用rhGM-CSF给小鼠灌胃高15倍以上;荧光显微镜成像表明:与轭合物充分混合的EGFP在37℃条件下的人工消化液中比未结合轭合物时更加稳定。因此,可以推断,家蚕蛹中30 ku蛋白群与壳聚糖的轭合物能提高蛋白质的口服吸收利用度,并能最大限度降低对目的蛋白的降解效率。
关 键 词: 应用生物化学;蚕蛹粉;30 ku蛋白群;壳聚糖;人工消化液;稳定性
Title: In vitro & in vivo evaluation on the effects of 30 ku protein-chitosan conjugates on improving protein utilization of oral absorption
Author: JIANG Xiaofeng, YU Fei, FANG Tengfei, LIAO Bifeng, YANG Bingfei, ZHANG Yaozhou
Organization: School of Life Science, Zhejiang University; Tianjin International Joint Academy of Biotechnology and Medicine
Abstract: In order to increase the target protein utilization degree of oral absorption, this study established the preparation process standards of 30 ku proteins and chitosan in silkworm pupa powder. 30 ku protein-chitosan conjugates was used to wrap the recombinant human granulocyte macrophage colony stimulating factor (rhGM-CSF), and this mixture was lavaged to the mice for increasing the absorption of rhGM-CSF. The conjugates was also used to wrap enhanced green fluorescence protein (EGFP) for increasing the stability of EGFP in artificial digestion juice. The ELISA detection showed that the amount of rhGM-CSF in serum when the mice was lavaged with rhGM-CSF wrapped with 30 ku protein-chitosan conjugates was 15 times higher than that with rhGM-CSF alone. Fluorescent microscope imaging showed that the stability of EGFP mixtured with 30 ku protein-chitosan conjugates was higher than EGFP alone in artificial digestion juice at at 37℃. Therefore, we concluded that silkworm pupa powder 30 ku protein-chitosan conjugates can improve protein utilization of oral absorption, and also protect the target proteins to avoid being digested in digestion juice.
Key words: applied biochemistry; silkworm pupa powder; 30 ku proteins; chitosan; artificial digestion juice; stability
发表期数: 2013年3月第5期
引用格式: 姜啸风,于飞,方腾飞,等. 家蚕蛹中30 ku蛋白群与壳聚糖轭合物对提高蛋白质口服吸收利用度的实验研究[J]. 中国科技论文在线精品论文,2013,6(5):414-422.
 
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