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结核分支杆菌Rv2869c拓扑结构研究

发表时间:2013-03-15  浏览量:925  下载量:457
全部作者: 朱文军,戴竹青,邹曼,徐朗莱,徐冬青
作者单位: 南京农业大学生命科学院,农业部农业环境微生物重点实验室
摘 要: 结核分支杆菌(Mycobacterium tuberculosis,MTB)的Rv2869c膜蛋白是S2P(site-2 protease)家族成员之一,由Rv2869c基因编码,是一种膜内切割蛋白酶(intramembrane-cleaving proteases,I-Clips)。为明确结核分支杆菌S2P相关蛋白Rv2869c的拓扑结构,研究首先利用网上拓扑结构预测服务器预测Rv2869c的拓扑结构,并采用报告基因—细菌碱性磷酸酶(phosphatase, phoA)基因和绿色荧光蛋白(green fluorescent protein, GFP)基因融合法对Rv2869c的拓扑结构进行测定,结果表明:Rv2869c有4个跨膜区,其C端位于细胞周质空间内。该研究为结核病(tuberculosis,TB)的预防和治疗提供了一定的理论基础。
关 键 词: 微生物学;拓扑结构;报告基因;Rv2869c;结核分支杆菌
Title: Study on topology of the Rv2869c from Mycobacterium tuberculosis
Author: ZHU Wenjun, DAI Zhuqing, ZOU Man, XU Langlai, XU Dongqing
Organization: Key Laboratory of Agricultural and Environmental Microbiology, Ministry of Agricultural, College of Life Sciences, Nanjing Agricultural University
Abstract: Rv2869c, a membrane protein of Mycobacterium tuberculosis (MTB), which belongs to S2P (site-2 protease) family, encoded by Rv2869c gene, is a kind of intramembrane-cleaving proteases (I-Clips). In order to obtain the topology of Rv2869c, an S2P homologue of MTB, the topology of Rv2869c was firstly predicted by web-based topology prediction server. Then the topology of Rv2869c was investigated using the reporter gene from the fusion of the bacterial alkaline phosphatase (phoA) gene and green fluorescent protein (GFP) gene. The results showed that Rv2869c harbored four transmembrane helices, and the C-terminal located in the periplasm. This research provides certain theoretical base for prevention and treatment of tuberculosis (TB).
Key words: microbiology; topology; reporter gene; Rv2869c; Mycobacterium tuberculosis
发表期数: 2013年3月第5期
引用格式: 朱文军,戴竹青,邹曼,等. 结核分支杆菌Rv2869c拓扑结构研究[J]. 中国科技论文在线精品论文,2013,6(5):423-430.
 
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