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锐孔法制备暗孢耳霉菌丝微胶囊的研究

发表时间:2013-03-15  浏览量:1104  下载量:296
全部作者: 陈相波,王丹琪,陈春
作者单位: 中国计量学院生命科学学院,浙江省生物计量及检验检疫技术重点实验室;浙江大学生命科学学院
摘 要: 实验对利用锐孔法制备暗孢耳霉(Conidiobolus obscurus)菌丝微胶囊进行研究。通过不同浓度的海藻酸钠溶液、不同浓度的CaCl2溶液、不同菌丝稀释倍数、不同型号针头和气流喷射压力等条件的正交实验,表明锐孔法制备暗孢耳霉菌丝微胶囊的最适实验条件为:海藻酸钠溶液浓度为4%,CaCl2溶液浓度为4%,菌丝稀释倍数为1倍,针头孔径为0.05 mm,气流压力为0.05 MPa. 研究为促进虫霉目真菌剂型化、利用虫霉目真菌进行生物防治打下了基础。
关 键 词: 微生物学;锐孔法;微胶囊;暗孢耳霉;杀虫真菌
Title: Preparation of microencapsulation of Conidiobolus obscurus mycelium using air-flow piercing device
Author: CHEN Xiangbo, WANG Danqi, CHEN Chun
Organization: Zhejiang Provincial Key Laboratory of Biometrology and Inspection & Quarantine Technique, College of Life Sciences, China Jiliang University; College of Life Sciences, Zhejiang University
Abstract: This paper studied the preparation of microencapsulation of Conidiobolus obscurus mycelium using air-flow piercing device. Through the orthogonal test on sodium alginate concentrations, CaCl2 solution concentration, mycelium dilution and pin types and air-flow pressure, the results showed that optimum experimental conditions for using air-flow piercing device to prepare mycelium microcapsulation were 4% sodium alginate, 4% CaCl2, one fold dilution of mycelium, 0.05 mm pin diameter and 0.05 MPa air pressure. The data provided foundation for formulation of Entomophthoralean fungus and pratice for its biological control.
Key words: microbiology; air-flow piercing device; microcapsulation; Conidiobolus obscurus; entomopathogenic fungi
发表期数: 2013年3月第5期
引用格式: 陈相波,王丹琪,陈春. 锐孔法制备暗孢耳霉菌丝微胶囊的研究[J]. 中国科技论文在线精品论文,2013,6(5):431-437.
 
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