您的位置:首页  > 论文页面

生物表面活性剂产生菌的分离与鉴定

发表时间:2013-03-15  浏览量:1076  下载量:621
全部作者: 左豫虎,毕思宁,侯巨梅,刘铜
作者单位: 黑龙江八一农垦大学农学院
摘 要: 采用富集培养、传代培养、血平板、油平板等方法从270份大庆油田的油土样中初步筛选出10株潜在的产生物表面活性剂菌株,通过进一步的排油法和乳化性能(E24)复筛获得一株产生物表面活性剂的优良菌株。对该菌株的生理生化特征、菌体和菌落形态学特征进行分析,并结合16S rDNA测定,表明该菌株属于假单胞菌属(Pseudomonas sp.),命名为假单胞杆菌BS1.
关 键 词: 应用微生物学;生物表面活性剂;血平板;乳化性能;假单胞杆菌属
Title: Isolation and identification of microbe producing biosurfactant
Author: ZUO Yuhu, BI Sining, HOU Jumei, LIU Tong
Organization: College of Agronomy, Heilongjiang Bayi Agricultural University
Abstract: Ten microbes which can produce biosurfactant possibly were isolated from 270 Daqing oil-contaminated soils by methods of enrichment culture, subculture, hemolytic activity assay on blood agar plates and hydrolyzing oil activity on oil agar plates. Then one excellent isolate as potential biosurfactant producer was obtained by degreasing oil assay and the emulsification index (E24). This isolate was identified as Pseudomonas sp. based on its physiological characteristics and analysis of its 16S rRNA sequence and was named as BS1.
Key words: applied microbiology; biosurfactant; blood agar plate; emulsification index; Pseudomonas sp.
发表期数: 2013年3月第5期
引用格式: 左豫虎,毕思宁,侯巨梅,等. 生物表面活性剂产生菌的分离与鉴定[J]. 中国科技论文在线精品论文,2013,6(5):438-442.
 
0 评论数 0
暂无评论
友情链接