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不同类群动物胰蛋白酶结构特征与酶学性质关系

发表时间:2013-03-15  浏览量:1149  下载量:395
全部作者: 王军仁,姜国良,吴志强
作者单位: 中国海洋大学海洋生命学院
摘 要: 选取Genebank中已知的完整胰蛋白酶(EC 3.4.21.4)序列,对昆虫胰蛋白酶(insect trypsin, I-TRY)、温热带甲壳动物胰蛋白酶(temperate and tropical zone crustacean trypsin, TTC-TRY)、冷水性鱼类胰蛋白酶(cold adapted fish trypsin, CAF-TRY)、温热带鱼类胰蛋白酶(temperate and tropical zone fish trypsin, TTF-TRY),以及高等脊椎动物胰蛋白酶(higher vertebrate trypsin, HV-TRY)的结构特征和酶学性质进行比较。结果表明:作为无脊椎动物和脊椎动物进化地位最高的2个类群,节肢动物和脊椎动物胰蛋白酶氨基酸数目、分子直径、结构多样性、酶功能多样性进化趋势存在差别。动物胰蛋白酶活性部位及附近大多数氨基酸侧链严格保守。由于外周和活性中心部位附近精氨酸(Arg)、谷氨酸(Glu)、天冬氨酸(Asp)、组氨酸(His)、半胱氨酸(Cys)、丙氨酸(Ala)、脯氨酸(Pro)、酪氨酸(Lyr)和甲硫氨酸(Met)的不同,不同类群动物胰蛋白酶的稳定性、柔性和反应耗能量差异显著,CAF-TRY,TTF-TRY和TTC-TRY催化常数(Kcat)升高或米氏常数(Km)降低,催化效率按下列顺序依次降低:CAF-TRY>TTF-TRY>TTC-TRY>I-TRY,HV-TRY.
关 键 词: 酶学;胰蛋白酶;结构特征;酶学性质
Title: Relationship of enzymologic properties with structure features of trypsin from different animal groups
Author: WANG Junren, JIANG Guoliang, WU Zhiqiang
Organization: College of Marine Life Sciences, Ocean University of China
Abstract: The reported trypsin sequences (EC 3.4.21.4) in Genebank were selected, and relationship between the structure features and enzymologic properties in insect trypsin (I-TRY), temperate and tropical zone crustacean trypsin (TTC-TRY), cold adapted fish trypsin (CAF-TRY), temperate and tropical zone fish trypsin (TTF-TRY) and higher vertebrate trypsin (HV-TRY) was comparied. It was found that, the evolutionary trend between the nummbers of amino acids, molecular diameter, diversity of molecular structure and fuction of arthropods and vertebrates trypsins were different in two groups with highest evolutionary position within invertebrates and chordates separately. The animal trypsin active centre and amino acid side chains round were strictly conservative. With differences of Arg, Glu, Asp, His, Cys, Pro, Ala, Tyr and Met around periphery and active centre, the stability, flexibility and peaction energy consumption of animal trypsin were significantly different. The Kcat of CAF-TRY, TTF-TRY and TTC-TRY was increased, while the Km of them was reduced. The catalytic efficiency was the following order: CAF-TRY>TTF-TRY>TTC-TRY>I-TRY and HV-TRY.
Key words: enzymology; trypsin; structure feature; enzymologic property
发表期数: 2013年3月第5期
引用格式: 王军仁,姜国良,吴志强. 不同类群动物胰蛋白酶结构特征与酶学性质关系[J]. 中国科技论文在线精品论文,2013,6(5):443-454.
 
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