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H2O2促进氯化钆诱导HepG2细胞凋亡
发表时间：2013-04-15 浏览量：1440 下载量：469
| 全部作者： | 叶丽华，刘会雪，王夔，杨晓达 |
| 作者单位： | 北京大学医学部药学院 |
| 摘 要： | 研究H2O2对氯化钆（GdCl3）诱导HepG2细胞凋亡的影响。结果显示：H2O2能够显著促进Gd3+诱导细胞凋亡；用Western blotting检测凋亡相关蛋白，发现H2O2与Gd3+共同作用时，可导致明显的DNA修复酶(poly ADP-ribose polymerase, PARP)剪切，但对凋亡相关促分裂素原活化蛋白激酶(mitogen-activated protein kinases, MAPK)以及线粒体信号通路的激活较两者单独作用都弱；溶液中H2O2和Gd3+共同作用可导致小牛胸腺DNA的断裂损伤。因此，上述结果表明H2O2促进Gd3+杀伤HepG2肿瘤细胞的机制不是通过增强Gd3+启动的细胞信号实现，而可能是二者共同作用造成对DNA的损伤所致，这为抗肿瘤稀土化合物研究提供了新的思路，有关其化学和分子生物学机制值得深入研究。 |
| 关 键 词： | 生物无机化学；GdCl3；H2O2；细胞凋亡；癌症；HepG2 |
| Title： | Hydrogen peroxide promotes gadolinium-induced apoptosis of HepG2 cells |
| Author： | YE Lihua, LIU Huixue, WANG Kui, YANG Xiaoda |
| Organization： | School of Pharmaceutical Sciences, Peking University Health Science Center |
| Abstract： | The present work investigated the roles of H2O2 on gadolinium (GdCl3)-induced apoptosis of HepG2 cancer cell line. The experimental results revealed that H2O2 promoted gadolinium-induced HepG2 apoptosis significantly. Analysis of apoptotic relevant proteins with Western blotting indicated that incubation of cells with (Gd3+) and H2O2 resulted in significant cleavage of poly ADP-ribose polymerase (PARP) proteins, but caused much weaker effects on mitogen-activated protein kinases (MAPK) or mitochondrial apoptotic signal transduction. Meanwhile, combination of (Gd3+) with hydrogen peroxide could cause fragmentations of calf thymus DNA. All these results suggested that, instead of enhancing Gd-induced cellular signal, H2O2 promoted HepG2 apoptosis by increasing Gd-induced DNA damages. Therefore, this mechanism might provide a new insight for design and discovery of anticaner lanthanide compounds, further works on the chemistry and molecular biology mechanisms would be appropriate. |
| Key words： | bio-inorganic chemistry; GdCl3; H2O2; apoptosis; cancer; HepG2 |
| 发表期数： | 2013年4月第7期 |
| 引用格式： | 叶丽华，刘会雪，王夔，等. H2O2促进氯化钆诱导HepG2细胞凋亡[J]. 中国科技论文在线精品论文，2013，6（7）：645-650. |
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