您的位置:首页  > 论文页面

肠道病毒71型重症患儿分离株SDLY96的全基因组序列分析

发表时间:2013-06-15  浏览量:1080  下载量:581
全部作者: 司鲁莹,温红玲,高峰,赵丽,宋艳艳,许洪芝,袁晓晶,王志玉
作者单位: 山东大学公共卫生学院,实验畸形学教育部重点实验室;山东省临沂市人民医院感染科
摘 要: 目的:对肠道病毒71型(Enterovirus 71,EV71)重症患儿分离株SDLY96进行全基因组序列测定与分析,为构建EV71感染性克隆奠定基础。方法:利用RT-PCR对2010年山东临沂1例手足口病(hand-foot-and-mouth disease, HFMD)重症患儿标本分离到的EV71型分离株(SDLY96)进行全基因组核苷酸序列测定,并对基因组序列进行同源性比较及遗传进化分析。结果:SDLY96株基因组全长7 405个核苷酸,SDLY96株与Fuyang株EU703813的核苷酸及氨基酸序列同源性均最高,整个基因组核苷酸同源性为98.4%,氨基酸同源性为99.1%. 基于VP1区的基因遗传进化分析显示,SDLY96株与Fuyang株EU703813进化关系最为接近。结论:SDLY96株属EV71病毒的C4基因亚型的C4a分支,与Fuyang株的核苷酸及氨基酸序列同源性很高。
关 键 词: 卫生检验学;肠道病毒71型;序列分析;全基因组
Title: Sequence analysis on complete genome of an Enterovirus 71 strain SDLY96 isolated from a severe case
Author: SI Luying, WEN Hongling, GAO Feng, ZHAO Li, SONG Yanyan, XU Hongzhi, YUAN Xiaojing, WANG Zhiyu
Organization: Key Laboratory of Experimental Teratology of Ministry of Education, School of Public Health, Shandong University; Department of Infectious Diseases, Linyi People’s Hospital
Abstract: Objective: To analyze the complete genome sequence of an Enterovirus 71 (EV71) strain SDLY96 isolated from a severe case and lay the foundation for constructing infectious clone of EV71. Methods: The complete genomes of SDLY96 from a sufferer of hand-foot-and-mouth disease (HFMD) in Linyi city of Shandong province were amplified by RT-PCR. The homology of nucleotide and amino acid sequences were aligned, and phylogenetic relations of the sequences were analyzed. Results: The full length of SDLY96 strains was 7 405 bp. SDLY96 strain shared the high homology with Fuyang strain EU703813 both in nucleotide and in amino acid sequences, with 98.4% identity in nucleotides and 99.1% in amino acids. The full VP1 region based phylogenetic analysis also indicated that strain SDLY96 had the close genetic relationship with Fuyang strain EU703813. Conclusion: SDLY96 should be classified as C4a branch of subgenogroup C4 of EV71, and SDLY96 strain has close genetic relationship with Fuyang strain.
Key words: sanitary technology; Enterovirus 71; sequence analysis; complete genome
发表期数: 2013年6月第11期
引用格式: 司鲁莹,温红玲,高峰,等. 肠道病毒71型重症患儿分离株SDLY96的全基因组序列分析[J]. 中国科技论文在线精品论文,2013,6(11):1014-1020.
 
0 评论数 0
暂无评论
友情链接