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脐带间充质干细胞成骨方向分化的研究

发表时间:2013-06-15  浏览量:1209  下载量:374
全部作者: 余永春,王伟新,孙洁,刘磊
作者单位: 四川大学口腔疾病国家重点实验室;广州医学院附属第一医院口腔科;新乡医学院第一附属医院口腔科;广东省口腔医院正畸科
摘 要: 目的:探讨脐带间充质干细胞向成骨细胞方向分化的潜能,为骨组织工程提供一种丰富的干细胞来源。方法:胰酶和胶原酶Ⅰ型联合消化法获得脐带间充质干细胞;免疫细胞化学法对其表面标志物进行鉴定;化学诱导法诱导脐带间充质干细胞向成骨细胞方向分化;倒置显微镜观察其形态变化;茜素红对诱导后的细胞染色。结果:胰酶和胶原酶Ⅰ联合消化法分离的细胞贴壁生长,呈现成纤维细胞形态;免疫细胞化学显示脐带间充质干细胞表达CD29,CD44和CD105,但不表达CD31,CD34和CD105;成骨诱导后的脐带间充质干细胞逐渐聚集生长,在聚集细胞中央形成结节样团块;茜素红染色显示结节为钙化物。结论:脐带间充质干细胞具有向成骨细胞方向分化的潜能,为骨组织工程提供了一种来源丰富、免疫力低和低分化的种子细胞。
关 键 词: 口腔颌面外科学;成骨分化;化学诱导;脐带间充质干细胞
Title: Study on osteogenic differentiation potential of umbilical cord mesenchymal stem cells
Author: YU Yongchun, WANG Weixin, SUN Jie, LIU Lei
Organization: State Key Laboratory of Oral Diseases, Sichuan University; Department of Stomatolgoy, First Affiliated Hospital of Guangzhou Medical College; Department of Stomatolgoy, First Affiliated Hospital of Xinxiang Medical College; Department of Orthodontics, Guangdong Provincial Stomatological Hospital
Abstract: Objective: To explore the osteogenic differentiation potential of umbilical cord mesenchymal stem cells and provide a rich source of stem cells for bone tissue engineering. Methods: Trypsin and collagenaseⅠdigestion method was used to obtain the umbilical cord mesenchymal stem cells. Surface markers were identified using immunocytochemical method. Chemical induction was used to induce umbilical cord mesenchymal stem cells into osteoblasts. Inverted microscope was used to observe the morphological changes. Alizarin red staining was used to stain the induced cells to check the osteogenic differentiation. Results: The cells isolated from umbilical cord using trypsin and collagenaseⅠdigestion method showed the adherent growth and fibroblast morphology. Immunocytochemistry showed that the umbilical cord mesenchymal stem cells were positive for CD29, 40 CD44 and CD105 while negative for CD31, CD34 and CD105. Umbilical cord mesenchymal stem cells were accumulated into growth after osteogenic induction and the nodular clumps formed in the center of the gathering cells. Then the osteogenic differentiation was determined by the production of calcium deposite detected by alizarin red staining. Conclusion: The umbilical cord mesenchymal stem cells have the osteogenic potential, which can provide the seeding cells with an abundant source, low immunity, and poor differentiation for bone tissue engineering.
Key words: oral and maxillofacial surgery; osteogenic differentiation; chemical induction; umbilical cord mesenchymal stem cells
发表期数: 2013年6月第11期
引用格式: 余永春,王伟新,孙洁,等. 脐带间充质干细胞成骨方向分化的研究[J]. 中国科技论文在线精品论文,2013,6(11):1040-1045.
 
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