您的位置:首页  > 论文页面

影响棉花体胚发生与植株再生的主要因素

发表时间:2013-09-15  浏览量:1146  下载量:347
全部作者: 刘正杰,张园,王彦霞,王玉美,华金平
作者单位: 中国农业大学农学与生物技术学院,作物杂种优势研究与利用教育部重点实验室,作物遗传改良北京市重点实验室;湖北省农科院经济作物研究所
摘 要: 近年来,植物组织培养已经被用于转基因工程与遗传改良的重要平台,对于植物组织培养的利用依赖于高效再生体系的建立。与其他植物相比,棉花是体细胞胚胎发生与植株再生最困难的植物之一,棉花体细胞胚发生与植株再生受诸多因素影响。这里综述了各个因素条件对棉花体胚发生与植株再生的影响,指出基因型决定棉花体细胞胚胎发生和植株再生能力。但是,可以通过选择适宜外植体,调节培养基激素种类与配比,或改变培养基成分,包括氮源与碳源、无机盐离子等,提高棉花体胚发生与植株再生能力。同时,通过改变光照与温度等培养条件,并进行适当的胁迫也有助于棉花再生能力的提高。高效的成苗培养、嫁接及移栽方式有助于再生植株的成活。针对目前体胚发生相关动态变化的分子机理相关研究不够透彻,以及棉花组织培养深入研究发展缓慢的现状,提出今后应该重点分析棉花胚性愈伤诱导及体胚发生阶段的基因表达及其分子机理。
关 键 词: 农艺学;棉花;综述;体细胞胚胎发生;植株再生;影响因素
Title: Main factors affecting somatic embryogenesis and plant regeneration in upland cotton
Author: LIU Zhengjie, ZHANG Yuan, WANG Yanxia, WANG Yumei, HUA Jinping
Organization: Key Laboratory of Crop Heterosis and Utilization of Ministry of Education, Beijing Key Laboratory of Crop Genetic Improvement, College of Agriculture and Biotechnology, China Agricultural University; Institute of Economic Crop, Hubei Academy of Agricultural Science
Abstract: Nowadays, plant tissue culture has been used as one of the technology platforms in transgenic engineering and genetic improvement. To set up an efficient regeneration system is the promise to the utilization of plant tissue culture. However, because of the many factors affecting somatic embryogenesis and subsequent plant regeneration, the tissue culture in cotton has dropped behind much comparing with other crops. This paper aims to make some summarization for the complicated factors influencing the somatic embryogenesis and plant regeneration of cotton, and points out that the genotype of material is the main factor influencing the capacity of somatic embryogenesis and plant regeneration, but this situation could be improved by selecting proper kinds of explants, adapting the kinds and proportion of plant hormones, and changing the composition of media, including nitrogen source and carbon source, inorganic ion. At the same time, using the proper illumination, temperature and adverse condition could promote the regeneration ability of cotton. The high efficiency system of seeding, grafting and transplant could largely contribute to the survivability of regeneration plants. Currently, the researches on molecular mechanism of dynamic changes related to somatic embryogenesis are not perceptive, and the in-depth study on tissue culture of cotton developments slowly. This review indicates that the researches should not only focus on the proper condition for tissue culture of cotton but also selectively analyze the molecular mechanism of dynamic changes of genes during the process of embryogenetic callus induction and the formation of somatic embryos.
Key words: agronomy; cotton; review; somatic embryogenesis; plant regeneration; affecting factors
发表期数: 2013年9月第17期
引用格式: 刘正杰,张园,王彦霞,等. 影响棉花体胚发生与植株再生的主要因素[J]. 中国科技论文在线精品论文,2013,6(17):1583-1592.
 
1 评论数 0
暂无评论
友情链接