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迟缓爱德华菌外膜蛋白图谱的建立及其在耐药研究中的意义

发表时间:2013-09-15  浏览量:1397  下载量:700
全部作者: 王超,彭宣宪,李惠
作者单位: 中山大学生命科学学院
摘 要: 为控制迟缓爱德华菌(Edwardsiella tarda)所引起的病害提供理论依据和防治指导,利用月桂酰肌氨酸钠的方法提取迟缓爱德华菌的外膜(outer membrane, OM)蛋白,并且建立双向电泳(two-dimensional electrophoresis, 2-DE)图谱。该图谱共有45个蛋白点,经过基质辅助激光解析电离飞行时间质谱(matrix-assisted laser desorption/lonization time of flight mass spectrometry, MALDI-TOF)鉴定,其中73.3%为外膜蛋白。抗生素滥用导致细菌耐药现象日益严重,因此利用2-DE图谱研究细菌外膜蛋白的耐药特点。首先通过传代培养筛选出LTB4菌株的四环素(tetracyline, TET)耐药菌,耐药倍数达到40倍,进而比较迟缓爱德华菌TET耐药菌株和出发菌株外膜蛋白的差异,共发现了6种与TET耐药相关的蛋白,分别是EvpB,LamB,OmpF2,ETAE-0245,EvpA和TolC,这些结果为深入开展迟缓爱德华菌对环境应激外膜蛋白质组学的研究奠定了扎实的基础。
关 键 词: 微生物学;迟缓爱德华菌;双向电泳;外膜蛋白;四环素
Title: Two-dimensional electrophoresis maps of outer membrane proteins in Edwardsiella tarda and the importance for the research of drug-resistance
Author: WANG Chao, PENG Xuanxian, LI Hui
Organization: School of Life Sciences, Sun Yat-sen University
Abstract: In order to provide theoretical and practical guide for control of Edwardsiella tarda infection, this paper extracted the outer membrane (OM) proteins of E.tarda using the sodium lauryl sarcosinate and established the two-dimensional electrophoresis (2-DE) maps. There were 45 protein spots on the 2-DE maps and 73.3% of them were classified into OM proteins by matrix-assisted laser desorption/lonization time of flight mass spectrometry (MALDI-TOF) analysis. As the bacterial drug resistance caused by antibiotics abuse is increasing by serious, the drug resistance features of OM proteins were studied by 2-DE maps. The tetracycline (TET) resistant LTB4 strain was screened out by subculturing first, the resistance index (RI) reached 40 times, Furthermore, six altered proteins on the 2-DE maps of OM proteins were identified in tetracycline(TET)-resistant strain in comparison with their original strains. They were EvpB, LamB, OmpF2, ETAE-0245, EvpA and TolC. These data provide useful information for further study of OM proteomics in response to environmental stresses.
Key words: microbiology; Edwardsiella tarda; two-dimensional electrophoresis; outer membrane protein; tetracycline
发表期数: 2013年9月第17期
引用格式: 王超,彭宣宪,李惠. 迟缓爱德华菌外膜蛋白图谱的建立及其在耐药研究中的意义[J]. 中国科技论文在线精品论文,2013,6(17):1607-1614.
 
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