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基于比较蛋白质组学筛选嗜酸氧化亚铁硫杆菌硫氧化相关蛋白质

发表时间:2013-09-15  浏览量:768  下载量:408
全部作者: 彭安安,夏金兰,刘红昌,朱薇,聂珍媛
作者单位: 中南大学资源加工与生物工程学院;中南大学生物冶金教育部重点实验室
摘 要: 在基于热水浴-Triton X-114相分离特化空间蛋白质的基础上,根据比较蛋白质组学方法和生物信息学分析,利用MALDI-TOF/MS技术和实时定量PCR技术,对嗜酸氧化亚铁硫杆菌(Acidithiobacillus ferrooxidans)ATCC 23270在元素硫和亚铁中培养的细胞胞外、外膜和周质空间蛋白质进行研究,筛选与硫氧化密切相关的关键蛋白。获得了39个与硫氧化相关的蛋白质,其中含有半胱氨酸残基的蛋白质约占70%,并有部分蛋白质中半胱氨酸丰度较高,含有1~2个—CXXC—结构域。半胱氨酸残基上的巯基在硫氧化过程中具有重要的活性功能,位于细菌细胞包被中这些富含半胱氨酸的蛋白质的鉴出,表明这些蛋白质在元素硫的活化-氧化过程中具有重要作用。
关 键 词: 生物工程;嗜酸氧化亚铁硫杆菌;比较蛋白质组学;硫氧化;生物信息学
Title: Isolation of sulfur oxidation relevant proteins of Acidithiobacillus ferrooxidans based on comparative proteomics
Author: PENG Anan, XIA Jinlan, LIU Hongchang, ZHU Wei, NIE Zhenyuan
Organization: School of Minerals Processing and Bioengineering, Central South University; Key Laboratory of Biometallurgy of Ministry of Education, Central South University
Abstract: Based on water-bathing-Triton X-114 phase separation of the cellular compartment-specific proteins, and the comparative proteomics and bioinformatic analysis, the extracellular, out-membrane and periplasmic space proteins were studied to isolate the key proteins relating to the sulfur oxidation of Acidithiobcillus ferrooxidans ATCC 23270 that were cultured on substrates elemental sulfur and ferrous iron, respectively. 39 expression upregulated proteins selected in the 2-DE profiles were identified by MALDI-TOF/MS and verified at transcriptional level by real-time quantitative PCR (real-time qPCR). 70% of which contained cysteine residues, some had higher cysteine content and one to two —CXXC— structural domains. This group of cysteine has important function in sulfur oxidation, the identification of the cell envelope proteins rich-in cysteine indicates these proteins play a key role in activation and oxidation of elemental sulfur.
Key words: bioengineering; Acidithiobacillus ferrooxidans; comparative proteomics; sulfur oxidation; bioinformatics
发表期数: 2013年9月第17期
引用格式: 彭安安,夏金兰,刘红昌,等. 基于比较蛋白质组学筛选嗜酸氧化亚铁硫杆菌硫氧化相关蛋白质[J]. 中国科技论文在线精品论文,2013,6(17):1615-1627.
 
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