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长期有氧运动对大鼠心肌p70S6K和4EBP1表达的影响

发表时间:2013-09-15  浏览量:1114  下载量:445
全部作者: 廖静雯,曾凡星,李奕,吴迎
作者单位: 北京体育大学运动人体科学学院
摘 要: 目的:观察p70核蛋白体S6激酶(p70S6K)和真核翻译起始因子4E结合蛋白(4EBP1)信号分子在长期有氧运动所致心肌肥大中的变化特点,以期为运动对心肌肥大的影响及相关蛋白合成的机制提供更多的理论支持。方法:7周龄雄性SD(Sprague-Dawley)大鼠30只随机分为安静对照组和有氧运动组,其中运动组分为4个取材点组,每组6只。运动干预采用28 m/min,60 min跑台运动方案,训练7周,每周训练5 d,周六、周日休息。每周一及最后取材前称体重,取心脏测其重量并分离左心室肌。Western blotting测定p70S6K,4EBP1的磷酸化及蛋白表达情况。结果:有氧运动组心系数与对照组相比具有非常显著性差异;有氧运动组p70S6K及4EBP1蛋白含量与安静组相比均无显著性差异;磷酸化p70S6K(Thr389)与对照组相比,在运动后即刻和6 h无显著性差异,在12 h达到峰值且有非常显著性差异,运动后24 h仍有显著性差异,未恢复至安静水平;运动组磷酸化的4EBP1(Thr37/46)在运动后即刻达到峰值,峰值显著高于安静时水平,随着时间逐渐减少,其后的6,12,24 h均低于安静水平,并在运动后24 h非常显著地低于安静时的磷酸化表达量。结论:长时间有氧运动引起了心肌肥大,p70S6K和4EBP1信号分子在中等强度组心肌肥大中发挥了作用,磷酸化4EBP1(Thr37/46)需要更多的实验和取材时间以确定其在运动后的变化特点。
关 键 词: 运动人体科学;有氧运动;Western blotting;心肌肥大;p70S6K;4EBP1
Title: Effects of long time aerobic exercises on the expressions of p70S6K and 4EBP1 in rat cardiac muscle
Author: LIAO Jingwen, ZENG Fanxing, LI Yi, WU Ying
Organization: Sport Science College, Beijing Sport University
Abstract: Objective: In the current study, the changing characteristics of p70S6K and 4EBP1 were focused on after aerobic exercise trainings of long period to explore the influences of long time exercise training on myocardial hypertrophy. Methods: 30 male Sprague-Dawley (SD) rats aged 7 weeks were randomly divided into 5 groups, 6 for each. The group control and aerobic exercise groups with myocardium being isolated at four different time points after the last exercise training. The training program lasted for 7 weeks with two days break each week. The protein levels of both the total and the phosphorylated forms of p70S6K and 4EBP1 were determined by Western blotting analysis. Results: The total amount of p70S6K and 4EBP1 of exercise training groups almost remains the same level as the control group. The phosphorylated p70S6K (Thr389) and its ratio to total p70S6K in the exercise group raise after the exercise and reach the peak value 12 hours after the last training. The exercise group’s amount of phosphorylated 4EBP1 (Thr37/46) and the ratio of 4EBP1 (Thr37/46)/4EBP1 reach their climax immediately after the last training and then diminish below the basal level. Conclusion: Long time aerobic exercise trainings have induced myocardial hypertrophy. p70S6K and 4EBP1 play important roles in the protein synthesis during the exercise training related to physiological myocardial hypertrophy, and more researches should be used to determine the characteristic of phosphorylated 4EBP1(Thr37/46).
Key words: sports science of human body; aerobic exercise; Western blotting; myocardial hypertrophy; p70S6K; 4EBP1
发表期数: 2013年9月第17期
引用格式: 廖静雯,曾凡星,李奕,等. 长期有氧运动对大鼠心肌p70S6K和4EBP1表达的影响[J]. 中国科技论文在线精品论文,2013,6(17):1677-1683.
 
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