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肌源性IL-6在胰岛素抵抗发生中的作用研究

发表时间:2013-09-15  浏览量:1214  下载量:505
全部作者: 雷雨,王萍,田丽,邢振超,文兵
作者单位: 铜仁学院体育系
摘 要: 目的:探讨肌源性IL-6在胰岛素抵抗发生中的作用。方法:大鼠正常饮食与高糖高脂饮食8周以形成对照。在此过程中采用8周跑台运动(第1周跑速为17 m/min,时间为50 min,随后每周跑速递增1 m/min,时间递增5 min)和/或注射IL-6 shRNA载体(休息日大鼠尾部静脉注射,每2周1次,共4次)干预,检测空腹胰岛素、空腹血糖及肝、腹脂、骨骼肌IL-6基因表达水平等指标。结果:大鼠高糖高脂饮食8周形成胰岛素抵抗;注射IL-6 shRNA组与对照组相比空腹胰岛素、空腹血糖显著增加(P<0.05,或P<0.01);注射IL-6 shRNA安静组大鼠肝、腹脂、骨骼肌IL-6基因表达水平显著下降;注射IL-6 shRNA运动组次之;高糖高脂运动组IL-6基因表达水平与安静高糖高脂组IL-6基因表达水平显著升高;运动注射IL-6 shRNA组血糖显著高于运动不注射IL-6 shRNA组(P<0.05,或P<0.01);8周有氧运动显著降低高糖高脂饮食鼠胰岛素抵抗发生的几率。结论:运动引起的肌源性IL-6具有抑制胰岛素抵抗发生的作用,但并不是唯一因素,且具有组织特异性。
关 键 词: 运动人体科学;IL-6;运动;胰岛素抵抗;作用
Title: Function of muscle-derived IL-6 on insulin resistance
Author: LEI Yu, WANG Ping, TIAN Li, XING Zhenchao, WEN Bing
Organization: Physical Education Department, Tongren University
Abstract: Objective: To study the role of muscle-derived IL-6 in insulin resistance occurence. Methods: The contrast experiments between normal diet and high glucose and fat diet were conducted in rats for 8 weeks. In this process, 8 weeks of treadmill exercise (the running speed was 17 m/min and time was 50 min in the first week, then running speed increased 1 m/min and time increased 5 min progressively every week) and/or injection of IL-6 shRNA vectors (intravenous injection into the rats’ tails on every two Monday, a total of 4 times) were used to intervent. The fasting insulin, fasting blood glucose and liver, abdominal fat, skeletal muscle IL-6 gene expression level and so on were tested. Results: Rats with high glucose and fat diet for 8 weeks have developed insulin resistance. Compared with the control group, fasting insulin and fasting blood glucose of injection of IL-6 shRNA group increased significantly (P<0.05, or P<0.01); liver, abdominal fat, skeletal muscle IL-6 gene expression level of injection of IL-6 shRNA quiet group decreased significantly; injection of IL-6 shRNA exercise group took second place. IL-6 gene expression level of both the high glucose and fat exercise group and the quiet high glucose and fat group was significantly increased; blood glucose of exercise injection of IL-6 shRNA group was significantly higher than that of not injection group (P<0.05, or P<0.01). 8 weeks aerobic exercise could significantly reduce the occurrence rate of insulin resistance in the high glucose and fat diet rats. Conclusion: Muscle-derived IL-6 caused by exercise can inhibit the action of insulin resistance, but not the only factor, which has tissue specificity.
Key words: sports science of human body; IL-6; exercise; insulin resistance; function
发表期数: 2013年9月第17期
引用格式: 雷雨,王萍,田丽,等. 肌源性IL-6在胰岛素抵抗发生中的作用研究[J]. 中国科技论文在线精品论文,2013,6(17):1684-1690.
 
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