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不同耐力训练量对兔股直肌肌球蛋白重链的影响

发表时间:2013-09-15  浏览量:1185  下载量:294
全部作者: 季苇,高维纬
作者单位: 北京体育大学运动康复系;北京体育大学运动医学教研室
摘 要: 目的:研究兔股直肌肌球蛋白重链(myosin heavy chain, MHC)各亚型在不同耐力训练量下的转化情况,探讨不同耐力训练量时兔股直肌MHC各亚型转化的规律。方法:45只雌性新西兰大白兔,按耐力训练量的不同随机分为G1,G2,G3,G4和G5组。兔左后肢作为实验侧进行耐力训练,右后肢为对照侧,无干预。各组完成规定运动量后,注射过量戊巴比妥钠处死、取材、测定蛋白含量、SDS-PAGE处理。用Image J图像处理软件对电泳得到的图像进行分析。采用SAS 6.1.2进行统计分析处理,显著性水平为P<0.05. 结果与结论:固定运动强度,随着耐力训练量的增加,MHC各亚型之间的转化首先是以MHC IIb→MHC IIa为主,然后变为MHC IIa→MHC I.
关 键 词: 运动人体科学;肌球蛋白重链;耐力训练;运动量;转化
Title: Effect of different endurance exercise amount on myosin heavy chain of rabbit rectus femoris
Author: JI Wei, GAO Weiwei
Organization: Sport Rehabilitation College, Beijing Sport University; The Section of Sports Medicine, Beijing Sport University
Abstract: Objective: The study was aimed at discussing the rule of different endurance exercise amount on myosin heavy chain (MHC) of rabbit rectus femoris. Methods: 45 adult female New Zealand rabbits according to different endurance exercise amount were randomly divided into G1, G2, G3, G4 and G5 group. Left legs were applied endurance exercise as the experimental side. Right legs were served as the control side. After finished the corresponding amount of endurance exercise, the rabbits were killed by using pentobarbital sodium over-dose, the rectus femoris muscles were sampled, protein content was tested by BCA method and MHC composition was assessed by using SDS-PAGE procedures. The pictures of MHC isoforms were analysed by Image J software. The statistical data were analysed by SAS 6.1.2 and the level of significance was for P<0.05. Results and Conclusion: These results demonstrate that under specific exercise intensity, with the increase in exercise amount, at first the major change is MHC IIb→MHC IIa, then the major change becomes MHC IIa→MHC I.
Key words: sports science of human body; myosin heavy chain; endurance exercise; exercise amount; transformation
发表期数: 2013年9月第17期
引用格式: 季苇,高维纬. 不同耐力训练量对兔股直肌肌球蛋白重链的影响[J]. 中国科技论文在线精品论文,2013,6(17):1691-1698.
 
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