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芸薹种SSR和UGMS标记遗传连锁图谱的构建

发表时间:2013-10-15  浏览量:1286  下载量:356
全部作者: 王哲,韩新丽,朴钟云
作者单位: 沈阳农业大学园艺学院
摘 要: 以芸薹种大白菜亚种多国芸薹属A基因组测序计划(multinational Brassica genome project, MBGP)模式植物“Chiifu”为父本,以小白菜亚种高代自交系“四九菜心”为母本,利用国际公用简单重复序列(simple sequence repeats, SSR)标记和本实验室设计开发的单基因微卫星标记(unigene microsatelite sequence, UGMS),以回交一代(first backcross,BC1)为作图群体,构建了一张总长度为1 159.9 cM、包含10条连锁群的分子遗传图谱。该图谱共计251个分子标记,其中包含120个UGMS标记和131个SSR标记。标记的平均图距为4.62 cM. 每条连锁群有15~37个标记,连锁群长度介于79.3~150.9 cM之间。该图谱为构建一张完整的大白菜染色体片段替换系奠定了基础。
关 键 词: 蔬菜学;芸薹种;简单重复序列标记;单基因微卫星标记;遗传图谱
Title: Construction of a genetic map of Brassica rapa based on SSR and UGMS marker
Author: WANG Zhe, HAN Xinli, PIAO Zhongyun
Organization: College of Horticulture, Shenyang Agriculture University
Abstract: Taking the model plant material of the multinational Brassica genome project (MBGP) “Chiifu” as donor parent and and an inbred line “sijiucaixin” as recurrent parent, a genetic map with with a total genome coverage of 1 159.9 cM and 10 linkage groups was constructed using simple sequence repeats (SSR) and unigene microsatelite sequence (UGMS), together with BC1 mapping population. This map was consisted of 251 polymorphic markers, including 120 UGMS and 131 public SSR markers. The average distance between adjacent loci was 4.62 cM. The number of markers on each linkage group was ranged from 15 to 37, and the length of linkage group from 79.3 cM to 150.9 cM. This map will lay a foundation for construction of chromosome segment substitution lines of Chinese cabbage.
Key words: vegetables; Brassica rapa; simple sequence repeat; unigene microsatellite sequence; genetic map
发表期数: 2013年10月第19期
引用格式: 王哲,韩新丽,朴钟云. 芸薹种SSR和UGMS标记遗传连锁图谱的构建[J]. 中国科技论文在线精品论文,2013,6(19):1814-1819.
 
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