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蛋白质磷酸化修饰对卵母细胞成熟的调控作用

发表时间:2013-12-15  浏览量:1085  下载量:525
全部作者: 肖正睿,张超,周涛,霍然,周作民,郭雪江
作者单位: 南京医科大学基础医学院;南京医科大学生殖医学国家重点实验室
摘 要: 目的:鉴定卵母细胞成熟过程中发生磷酸化修饰改变的蛋白质,并阐述磷酸化修饰的改变介导的蛋白质变化对卵母细胞成熟的调控作用。方法:利用凝胶分析软件比对卵母细胞成熟过程表达差异蛋白质图谱和MII期卵母细胞磷酸化修饰蛋白质图谱数据,找出卵母细胞成熟过程中发生磷酸化修饰改变的蛋白质,并利用生物信息学工具系统注释这些蛋白质的功能,分析其参与的调控网络。结果:通过分析鉴定到9种蛋白质在卵母细胞成熟过程中发生磷酸化修饰的改变,这些蛋白质大部分具有酶活性并组成复杂调控网络。结论:研究揭示了卵母细胞成熟过程中磷酸化修饰改变的蛋白质可能参与调控卵母细胞的成熟和早期胚胎发育,为进一步研究卵母细胞成熟和功能的调控机制奠定了基础。
关 键 词: 基础医学其他学科;蛋白质组学;磷酸化修饰;卵母细胞
Title: Protein phosphorylation in regulation of oocyte maturation
Author: XIAO Zhengrui, ZHANG Chao, ZHOU Tao, HUO Ran, ZHOU Zuomin, GUO Xuejiang
Organization: School of Basic Medical Science, Nanjing Medical University; State Key Laboratory of Reproductive Medicine, Nanjing Medical University
Abstract: Objective: To identify quantitatively the changed proteins which were modified with phosphorylation during oocyte maturation, and study the regulatory role of protein phosphorylation during oocyte maturation. Methods: By using gel electrophoresis image analysis software, the changed proteins with phosphorylation during oocyte maturation were identified from maps of differentially expressed proteins and phosphorylated proteins in MII oocyte. Furthermore, the bioinformatics tools were used to analyze the function and regulatory network of these proteins. Results: A total of 9 proteins were identified as phosphorylation changed proteins during oocyte maturation, most of them had catalytic activity and evolved in a complex regulatory network. Conclusion: This study reveals that protein phosphorylation change may play a role in the regulatory of oocyte maturation and early embryonic development, which provides a foundation for further research the mechanism of oocyte maturation and function.
Key words: other subjects of basic medicine; proteomics; phosphorylation; oocyte
发表期数: 2013年12月第23期
引用格式: 肖正睿,张超,周涛,等. 蛋白质磷酸化修饰对卵母细胞成熟的调控作用[J]. 中国科技论文在线精品论文,2013,6(23):2235-2240.
 
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