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新加良附方联合5-Fu对裸鼠胃癌移植瘤survivin/caspase-3表达的影响

发表时间:2013-12-15  浏览量:1041  下载量:299
全部作者: 宋延平,许晶,赵林涛,吴晓勇,王婧,侯丽
作者单位: 陕西省中医药研究院药理室;陕西中医学院药学院;贵阳中医学院第一附属医院肿瘤血液科;北京中医药大学东直门医院肿瘤血液科;北京中医药大学中医内科学教育部重点实验室,北京市重点实验室
摘 要: 目的:观察新加良附(Xinjialiangfu,XJLF)方联合5-氟尿嘧啶(5-Fu)对人胃癌细胞(SGC-7901)裸鼠移植瘤生长抑制作用及其对肿瘤组织中survivin及caspase-3蛋白表达的影响。方法:建立移植性人胃癌细胞(SGC-7901)裸鼠移植瘤模型,随机分为模型对照组,5-Fu组及XJLF方高、中、低剂量组,联合给药组(XJLF中剂量+5-Fu组)等6组。连续给药10 d,计算肿瘤抑制率,检测survivin/caspase-3蛋白的表达。结果:联合给药组抑瘤率为60.28%,与模型对照组比较,有统计学意义(P<0.05);与5-Fu组和XJLF高剂量组比较,联合给药组抑瘤率升高明显,有统计学意义(P<0.05);联合给药组上调肿瘤组织caspase-3蛋白表达,下调survivin蛋白表达,与模型对照组比较,有统计学意义(P<0.05,P<0.01);与5-Fu组和XJLF高剂量组比较,联合给药组caspase-3蛋白表达明显升高(P<0.05),survivin蛋白表达明显下降(P<0.05)。结论:联合给药组抑瘤率高于XJLF高剂量组及5-Fu组,在调节caspase-3,survivin表达方面优于XJLF高剂量组及5-Fu组,显示了两药协同作用的优势。
关 键 词: 中药学;新加良附方联合5-Fu;人胃癌细胞(SGC-7901);survivin;caspase-3
Title: Effects of Xinjialiangfu formula and 5-Fu combination on survivin and caspase-3 expression of gastric cancer xenografts in nude mice
Author: SONG Yanping, XU Jing, ZHAO Lintao, WU Xiaoyong, WANG Jing, HOU Li
Organization: Department of Pharmacology, Shaanxi Institute of Traditional Chinese Medicine; College of Pharmacy, Shaanxi University of Traditional Chinese Medicine; Department of Hematology Oncology, The First Affiliated Hospital of Guiyang College of Traditional Chinese Medicine; Department of Hematology Oncology, Dongzhimen Hospital of Beijing University of Chinese Medicine; Key Laboratory of Chinese Internal Medicine, Ministry of Education and Beijing City, Beijing University of Chinese Medicine
Abstract: Objective: To observe the effect of Xinjialiangfu (XJLF) formula on human gastric cancer (SGC-7901) tumor growth in nude mice and its impact on survivin and caspase-3 expression in tumor tissue. Methods:The animal model of SGC-7901 xenografted nude mice was established, and all the mice were randomly divided into 6 groups namely model control group, 5-Fu group, XJLF formula high-dose group, XJLF formula medium-dose group, XJLF formula low-dose group and drug combination group (XJLF formula medium-dose and 5-Fu). After 10 d of continuous treatment, tumor inhibition rate was calculated and the expression of survivin and caspase-3 was detected by immunohistochemistry techniques. Results: The tumor inhibition rate of drug combination group was 60.28%, which was significantly increased compared to the model control group (P<0.05), 5-Fu group (P<0.05) and XJLF high-dose group (P<0.05), respectively. Meanwhile, the caspase-3 expression was significantly up-regulated and the survivin expression was significantly down-regulated in drug combination group, compared to the model control group (P<0.05,P<0.01), 5-Fu group (P<0.05,P<0.05) and XJLF formula high-dose group (P<0.05,P<0.05), respectively. Conclusion: The tumor inhibition rate of drug combination group is higher than XJLF formula high-dose group and 5-Fu group. Also combination group has stronger effect compared to model control group in terms of own-regulation of caspase-3 expression and up-regulation of survivin expression, which indicates a benefit of XJLF and 5-Fu combination treatment.
Key words: science of Chinese pharmacology; Xinjialiangfu formula and 5-Fu combination; human gastric cancer (SGC-7901); survivin; caspase-3
发表期数: 2013年12月第23期
引用格式: 宋延平,许晶,赵林涛,等. 新加良附方联合5-Fu对裸鼠胃癌移植瘤survivin/caspase-3表达的影响[J]. 中国科技论文在线精品论文,2013,6(23):2259-2264.
 
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