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补肝汤调控杏仁核恐惧关键基因Egr-1和GRP表达的研究

发表时间:2013-12-15  浏览量:1158  下载量:394
全部作者: 徐世军,代渊
作者单位: 成都中医药大学药学院,四川省中药资源系统研究与开发利用国家重点实验室培育基地;成都中医药大学基础医学院
摘 要: 目的:考察补肝经方补肝汤对震后惊恐障碍(post-earthquake panic disorder,PEPD)模型大鼠杏仁核生长反应基因1 (earlygrowth response gene 1,Egr-1)和胃泌素释放肽(astrin-releasing peptide,GRP)基因表达的影响,为补肝汤治疗PEPD提供实验依据。方法:单程长时刺激(single prolonged stress, SPS)法制备创伤后应激障碍(post-traumatic stress disorder,PTSD)大鼠模型,补肝汤干预7 d后,处死大鼠,无菌条件下取杏仁核,采用荧光定量PCR法检测杏仁核中Egr-1和GRP相对表达量。结果:PTSD模型大鼠杏仁核Egr-1和GRP较空白对照组显著高表达(P<0.05);补肝汤高、中剂量组杏仁核Egr-1和GRP相对表达量均较模型对照组显著降低(P<0.05)。结论:补肝汤能抑制惊恐障碍大鼠杏仁核中Egr-1和GRP的异常高表达,说明补肝汤抗惊恐障碍与抑制杏仁核恐惧关键基因Egr-1和GRP的表达有关。
关 键 词: 中药药理学;补肝汤;惊恐障碍;生长反应基因1;胃泌素释放肽基因
Title: Effect of Bugan decorction on expression of Egr-1 and GRP of the amygdala in rats with post-earthquake panic disorder
Author: XU Shijun, DAI Yuan
Organization: Sichuan Provincial Key Laboratory of Systematic Research and Development of Chinese Medicina Resources, Province-Ministry Co-constructed State Key Lab Incubation Base, College of Pharmacy; College of Basic Medical, Chengdu University of TCM
Abstract: Objective: To analyze the effect of the Bugan decorction on post-earthquake panic disorder (PEPD), and explore the mechanism of this effect on express of early growth response gene 1 (Egr-1) and gastrin-releasing peptide (GRP) in amygdala with panic disorder. Methods: The post-traumatic stress disorder (PTSD) rat model was made by single prolonged stress (SPS), and the rats were killed after the treatment with Bugan decorction for 7 days. Then, the scared central amygdale sample was made in the sterile condition, the real-time PCR was used to determine the expression of the amygdala Egr-1 and GRP in the rats’ amygdaloid nuclear. Results: Compared to the blank control group, the expression of Egr-1 and GRP increased significantly (P<0.05). However, compared with the model group, the treatment of high and medium Bugan decorction decreased the expression of Egr-1 and GRP increased significantly (P<0.05). Conclusion: Bugan decorction can be used to resist the panic disorder by increasing the synthesis of the central amygdaloid brain nuclear Egr-1 and GRP.
Key words: pharmacology of traditional Chinese medicine; Bugan decorction; panic disorder; earlygrowth response gene 1; astrin-releasing peptide gene
发表期数: 2013年12月第23期
引用格式: 徐世军,代渊. 补肝汤调控杏仁核恐惧关键基因Egr-1和GRP表达的研究[J]. 中国科技论文在线精品论文,2013,6(23):2265-2269.
 
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