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拟南芥AtAGP11基因超表达导致植株败育的研究

发表时间:2014-03-15  浏览量:1471  下载量:627
全部作者: 钟静,张雪莲,赵洁
作者单位: 武汉大学杂交水稻国家重点实验室,生命科学学院
摘 要: 运用花粉和胚胎的分离技术、DAPI染色技术、人工授粉杂交技术和半薄切片技术,并结合生物统计学方法对AtAGP11超表达转基因植株进行表型观察,发现在AtAGP11超表达植株中存在较为严重的败育现象。进一步的研究结果显示:AtAGP11超表达抑制了花丝细胞的生长,引起花丝变短,导致花粉无法授于柱头上而产生植株败育。研究发现:过量表达AtAGP11基因影响了花丝细胞的正常生长,这表明该基因可能在细胞生长过程中起着重要的作用。
关 键 词: 植物发育学;拟南芥;AtAGP11基因;超表达;败育
Title: Over-expression of AtAGP11 gene leads to abortion in Arabidopsis thaliana
Author: ZHONG Jing, ZHANG Xuelian, ZHAO Jie
Organization: State Key Laboratory of Hybrid Rice, College of Life Sciences, Wuhan University
Abstract: In this paper, we adopted pollen and embryo separation, pollen DAPI dyeing, artificial pollination, semi-thin section technologies and biostatistics to observe the phenotypes of AtAGP11 over-expression transgenic plants and found serious abortion phenomenon in the AtAGP11 over-expression plants. Further research showed that over-expression of AtAGP11 affected pollination by shortening the length of filaments so that pollen could not land on stigma, which resulted in abortion phenotype. This study discovered that over-expression of AtAGP11 affected regular growth of filaments, indicating a key role of AtAGP11 in cell growth.
Key words: plant developmental biology; Arabidopsis thaliana; AtAGP11 gene; over-expression; abortion
发表期数: 2014年3月第5期
引用格式: 钟静,张雪莲,赵洁. 拟南芥AtAGP11基因超表达导致植株败育的研究[J]. 中国科技论文在线精品论文,2014,7(5):417-427.
 
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