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FISH检测miRNA-34c在小鼠睾丸和精原干细胞的表达

发表时间:2014-03-15  浏览量:1238  下载量:345
全部作者: 于萌,刘维帅,华进联
作者单位: 西北农林科技大学动物医学院,陕西省干细胞工程技术研究中心,农业部动物生物技术重点实验室;杨凌示范区医院病理科
摘 要: 目的:研究miRNA-34c(miR-34c)在小鼠睾丸组织和小鼠精原干细胞(spermatogonial stem cells,SSCs)中的表达规律。方法:应用荧光原位杂交(fluorescence in situ hybridization,FISH)技术完成在睾丸组织切片和SSCs中的miR-34c示踪。结果:miR-34c在2月龄小鼠睾丸中表达量较高;在转染入miR-34c 的模拟物组中,miR-34c表达量较其他组高。结论:使用FISH方法探索miRNA表达规律较稳定,且重复率高。
关 键 词: 细胞生物学;荧光原位杂交;精原干细胞;睾丸;小鼠
Title: Identification of miRNA-34c expression in mouse testis and spermatogonial stem cells by FISH
Author: YU Meng, LIU Weishuai, HUA Jinlian
Organization: Key Lab for Animal Biotechnology of Agriculture Ministry of China, Shaanxi Centre of Stem Cells Engineering & Technology, College of Veterinary Medicine, Northwest A&F University; Department of Patholohy, Yangling Demonstration Hospital
Abstract: Objective: To explore the expression of miRNA-34c (miR-34c) in mouse testis tissues and mouse spermatogonial stem cells (SSCs). Methods: The technology of fluorescence in situ hybridization (FISH) was used to illustrate the miR-34c expression in mouse testis tissues and SSCs. Results: The expression of miR-34c was higher in 2 month old mouse testis tissue slices. Furthermore, the expression of miR-34c was higher in the group of miR-34c-transfected mimics. Conclusion: FISH is a stable and repeatable method in measuring miRNA expression.
Key words: cell biology; fluorescence in situ hybridization; spermatogonial stem cell; testis; mouse
发表期数: 2014年3月第5期
引用格式: 于萌,刘维帅,华进联. FISH检测miRNA-34c在小鼠睾丸和精原干细胞的表达[J]. 中国科技论文在线精品论文,2014,7(5):440-444.
 
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