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人类单纯疱疹病毒和无机汞协同作用的动物实验

发表时间:2014-06-15  浏览量:1708  下载量:561
全部作者: 熊天庆,谭百宏,李艳超
作者单位: 吉林大学白求恩医学院基础医学部;吉林大学白求恩医学院基础医学实验教学中心
摘 要: 人类单纯疱疹病毒1(human herpes simplex virus 1,HSV-1)感染和微量汞中毒极有可能共存于同一个体的脑内而发生协同作用。为验证这一假说,在14周C57BL/6小鼠双侧足垫皮下连续2 d注射75 μL 2 g/L HgCl2后,在足垫皮下注射75 μL HSV-1 (2×106 PFU). 注射后通过金属自显影检测汞的阳性细胞,并采用免疫荧光染色观察Tau蛋白和Tau磷酸化蛋白的表达情况。结果显示:小鼠足垫注射汞后,注射后第4天可在脊髓前角检测到阳性神经元;与对照组和汞注射组相比,Tau蛋白和磷酸化Tau蛋白在汞加HSV-1组的脊髓前角内表达显著增加。结果表明:汞与HSV-1可能存在某种协同机制,并且该协同机制能引起神经元退行性改变。
关 键 词: 神经病学;人类单纯疱疹病毒;无机汞;动物模型;Tau蛋白
Title: Study on the synergic action of human herpes simplex virus and inorganic mercury in animal model
Author: XIONG Tianqing, TAN Baihong, LI Yanchao
Organization: College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University; Laboratory Teaching Center of Basic Medicine, College of Basic Medical Sciences, Norman Bethune Health Science Center of Jilin University
Abstract: In order to investigate the possible synergic mechanism of inorganic mercury and human herpes simplex virus in the central nervous system, about 75 μL HSV-1 (2 × 106 PFU) and 75 μL 2 g/L HgCl2 was injected bilaterally into the footpads of 14-week old C57BL/6 for 2 d, then 75 μL HSV-1 (2×106 PFU) was injected into the footpads. The positive neurons for mercury were detected by autometallography. Immunofluorescence was used to reveal the changes of Tau protein and phosphorylation of Tau protein. The results showed that after footpad injection, mercury was found within the motoneurons in the spinal anterior horn after the 4th day of injection. As compared with the control group and mercury-injected group, Tau protein and phosphorylated Tau protein were significantly increased in the spinal cord in the group which was injected with both mercury and HSV-1. These findings suggested that there might be a synergic mechanism between mercury and HSV-1, which was related to the neurodegenerative changes in the central nervous system.
Key words: neurology; human herpes simplex virus; inorganic mercury; animal model; Tau protein
发表期数: 2014年6月第11期
引用格式: 熊天庆,谭百宏,李艳超. 人类单纯疱疹病毒和无机汞协同作用的动物实验[J]. 中国科技论文在线精品论文,2014,7(11):1055-1060.
 
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