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电离辐射对卵巢癌细胞SKVCR2.0多药耐药性的影响

发表时间:2014-06-15  浏览量:1719  下载量:534
全部作者: 徐珊,孔德娟,王洪智,陈海洋,马淑梅,刘晓冬
作者单位: 吉林大学公共卫生学院,卫生部放射生物学重点实验室;吉林大学公共卫生学院
摘 要: 目的:探讨不同电离辐射方式对卵巢癌多药耐药性(multi-drug resistance,MDR)的影响。方法:采用MTT法观察卵巢癌耐药细胞SKVCR2.0和卵巢癌细胞SKOV-3对长春新碱(vincristine,VCR)、顺铂(cis-dichlorodiamineplatinum(II),DDP)、吡柔比星(therarubicin,THP)的药物敏感性。选取VCR诱导的卵巢癌耐药细胞株SKVCR2.0给予照射。照射方案分为常规分割(2 Gy/次,1次/d)和超分割(1 Gy/次,2次/d,间隔4 h)2种,总剂量为6 Gy. 采用cell counting kit-8(CCK-8)法测量不同照射方案下卵巢癌耐药细胞SKVCR2.0的存活率;采用单丹磺酰戊二胺(monodansylcadaverine,MDC)法测量在不同照射方案下卵巢癌耐药细胞SKVCR2.0的自噬发生率。在上述基础上,加入自噬抑制剂三甲基腺嘌呤(three methyl adenine,3-MA)再测量其存活率和自噬发生率。结果:卵巢癌耐药细胞SKVCR2.0较卵巢癌细胞SKOV-3具有MDR. 随着VCR浓度的升高,卵巢癌耐药细胞株SKVCR2.0的细胞存活率降低,但给予照射后,细胞存活率升高,自噬发生率明显增加,且超分割较常规分割自噬发生率更高。加入自噬抑制剂3-MA后,2组细胞的自噬发生率均明显降低。结论:分割剂量电离辐射通过提高自噬水平促进肿瘤MDR.
关 键 词: 放射卫生学;卵巢癌细胞;电离辐射;多药耐药性
Title: Effect of irradiation on the multi-drug resistance of ovarian cancer cell SKVCR2.0
Author: XU Shan, KONG Dejuan, WANG Hongzhi, CHEN Haiyang, MA Shumei, LIU Xiaodong
Organization: Key Laboratory of Radiobiology, Ministry of Health, School of Public Health, Jilin University; School of Public Health, Jilin University
Abstract: Objective: To discuss the effect of irradiation on the multi-drug resistance (MDR) of ovarian cancer cells. Methods: MTT assay was used to detect the drug sensitivities of SKVCR2.0 and SKOV-3 cells in vincristine (VCR), cis-dichlorodiamineplatinum(II) (DDP) and therarubicin (THP) groups. We chose ovarian cancer cells SKVCR2.0 induced by VCR and then irradiate them. The methods of irradiation included conventional fraction (2 Gy/time, 1 time/d) and hyperfraction (1 Gy/time, 2 times/d, with 4 h interval). The total dose was 6 Gy. The viability of SKVCR2.0 cells was tested by cell counting kit-8 (CCK-8), and the incidence of autophagy of SKVCR2.0 cells was tested by monodansylcadaverine (MDC). Then, based on the above, the cell viability and the incidence of autophagy after adding three methyl adenine (3-MA) were tested. Results: Compared with SKOV-3, SKVCR2.0 had higher MDR. With the increase of VCR, the cell viability of SKVCR2.0 decreased, while after irradiation, cell survival increased and the incidence of autophagy increased significantly. Moreover, there was a higher incidence of autophagy in hyperfraction group. In addition, when adding 3-MA, the incidence of autophagy of the two groups decreased. Conclusion: Fractionated irradiation leads to MDR through increased the incidence of autophagy.
Key words: radiohygiene; ovarian cancer cell; irradiation; multi-drug resistance
发表期数: 2014年6月第11期
引用格式: 徐珊,孔德娟,王洪智,等. 电离辐射对卵巢癌细胞SKVCR2.0多药耐药性的影响[J]. 中国科技论文在线精品论文,2014,7(11):1080-1086.
 
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