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中华鳖附睾内SOD活力的季节性变化
发表时间：2014-06-15 浏览量：2110 下载量：639
| 全部作者： | 卞勋光，杨平，陈秋生 |
| 作者单位： | 南京农业大学动物医学院；盐城师范学院生命科学与技术学院 |
| 摘 要： | 为研究生殖周期内中华鳖附睾超氧化物歧化酶（superoxide dismutase，SOD）的变化，分别测定了生殖期（6月和8月）和冬眠期（12月和2月）附睾各段组织及管腔液内SOD的活力。结果显示，在整个生殖周期内，各段附睾液中SOD活力差异较大，且同一部位附睾液内SOD的活力生殖期高于冬眠期。生殖期内，尾部的附睾液SOD活力最高，为（1 518.53±124.88）U/mL，与头部和体部差异显著（P<0.05）。冬眠期内，头部附睾液SOD活力最高，为（508.45±45.40）U/mL，与体部和尾部差异显著（P<0.05）。生殖期附睾尾部组织中SOD的活力最高，为（137.58±14.81） U/mg蛋白，与附睾体部差异显著（P<0.05），但与附睾头部差异未达到显著水平（P>0.05）。冬眠期附睾组织中SOD的活力低于生殖期，且各段之间SOD的活力差异不显著（P>0.05）。附睾中SOD活力呈季节性变化，表明它可能与精子在附睾内的运动和储存具有一定相关性。 |
| 关 键 词： | 畜牧、兽医科学基础学科；中华鳖；附睾；超氧化物歧化酶 |
| Title： | Seasonal changes of SOD activity in the epididymis of the soft-shelled turtle (Pelodiscus sinensis) |
| Author： | BIAN Xunguang, YANG Ping, CHEN Qiusheng |
| Organization： | College of Veterinary Medicine, Nanjing Agricultural University; College of Life Science and Techndogy, Yancheng Teachers University |
| Abstract： | In order to study the variation of superoxide dismutase (SOD) in epididymis of soft-shelled turtle during the reproductive cycles, the SOD activity in both tissues and fluid in each segment of epididymis were measured during reproductive seasons (June and August) and hibernation seasons (December and February). The results showed that the SOD activity in epididymal fluid had varied among each segment of epididymis throughout the reproductive cycles, and was higher in reproductive seasons than that in hibernant seasons at same segment. It showed that the highest SOD activity in caudal epididymal fluid was (1 518.53�24.88) U/mL, extremely striking contrast with it in caput and corpus (P<0.05) during reproductive seasons. However, in hibernant seasons, the SOD activity in caput epididymal fluid was the highest with (508.45 �45.40) U/mL, and it was significant different with that in corpus and cauda (P<0.05). Among epididymal tissue, the SOD activity had little discrepancy, and had the highest level at cauda with (137.58�4.81) U/mg prot, which was significantly different with that in corpus (P<0.05), while no difference with that at caput (P>0.05) during reproductive seasons. The SOD activity in epididymal tissue had no significant contrast among each segments (P>0.05) in hibernation seasons, and was lower than that in reproductive seasons. In the epididymis, the SOD activity showed seasonal variation, suggesting that it might have a certain correlation with the movement and storage of sperm in the epididymis. |
| Key words： | basic subject of animal husbandry and veterinary medicine science; soft-shelled turtle; epididymis; superoxide dismutase |
| 发表期数： | 2014年6月第11期 |
| 引用格式： | 卞勋光，杨平，陈秋生. 中华鳖附睾内SOD活力的季节性变化[J]. 中国科技论文在线精品论文，2014，7（11）：1108-1111. |
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