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响应曲面法优化枯草芽孢杆菌(Bacillus subtilis)发酵产腺苷的研究

发表时间:2014-09-15  浏览量:1514  下载量:452
全部作者: 王世锋,何剑,高俊,郑涛,陈怡露,雍晓雨
作者单位: 南京工业大学生物与制药工程学院;南京工业大学生物能源与环境生物技术研究所
摘 要: 为提高枯草芽孢杆菌(Bacillus subtilis)M3-3的摇瓶发酵腺苷产量,运用响应曲面法优化腺苷摇瓶发酵条件。结果表明:当发酵温度为34℃、摇床转速为210 r/min、发酵时间为69.9 h时,腺苷产量最高,为14.39 g/L. 利用RT-PCR 检测腺苷代谢途径中关键基因guaB,purA和purB基因优化前后的表达差异,结果表明:相对于原始发酵条件,在优化条件下guaB基因的下调和purA,purB基因的上调能够导致该菌株的代谢流流向有利于腺苷积累的方向,从而提高腺苷产量。
关 键 词: 微生物学;发酵优化;腺苷;响应曲面法;RT-PCR
Title: Study on fermentative production of adenosine by Bacillus subtilis using response surface methodology
Author: WANG Shifeng, HE Jian, GAO Jun, ZHENG Tao, CHEN Yilu, YONG Xiaoyu
Organization: College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University; Bioenergy and Environmental Biotechnology Research Institute, Nanjing Tech University
Abstract: To improve adenosine production in Bacillus subtilis M3-3, response surface methodology was conducted on optimization of shake flask fermentation conditions. The results showed that the highest adenosine yield arrived at 14.39 g/L when the fermentation temperature was 34℃, rotate speed was 210 r/min and fermentation time was 69.9 h, respectively. RT-PCR was applied to determine the relative differences of gene-expression in adenosine biosynthesis pathway. It was found that down-regulation of guaB, up-regulation of purA and purB would make the metabolic flux flow to the direction of adenosine accumulation, which resulted in increase of adenosine production.
Key words: microbiology; fermentative optimization; adenosine; response surface methodology; RT-PCR
发表期数: 2014年9月第17期
引用格式: 王世锋,何剑,高俊,等. 响应曲面法优化枯草芽孢杆菌(Bacillus subtilis)发酵产腺苷的研究[J]. 中国科技论文在线精品论文,2014,7(17):1701-1708.
 
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