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北冰洋海水可培养微生物多样性分析

发表时间:2014-09-15  浏览量:2122  下载量:863
全部作者: 饶萌,杨季芳,陈吉刚
作者单位: 浙江万里学院生物与环境学院,宁波市微生物与环境工程重点实验室
摘 要: 北极科考利用温盐深仪(conductivity temperature depth, CTD)采集了B80-3.5 km,B79-100 m,C19-42 m 3个站点的北冰洋海水,应用“原位”透析室培养法对海水样品进行连续富集培养。用16S rDNA文库技术对3个站点可培养细菌多样性进行研究,结果显示微生物多样性丰富。B80-3.5 km站点归属于4个类群:α-变形菌纲(Alphaproteobacteria)66.66%,γ-变形菌纲(Gammaproteobacteria)21.21%,噬纤维菌纲(Cytophagia)4.04%,未知分类地位8.08%. B79-100 m站点归属于4个类群:α-变形菌纲83.64%,γ-变形菌纲10.20%,噬纤维菌纲3.06%,未知分类地位3.06%. C19-42 m站点归属于4个类群:α-变形菌纲69.69%,γ-变形菌纲12.12%,噬纤维菌纲5.05%,未知分类地位13.13%.
关 键 词: 海洋微生物学;北冰洋海水;透析室富集培养;16S rDNA文库;海洋微生物多样性
Title: Analysis of culturable bacterial community composition from Arctic seawater
Author: RAO Meng, YANG Jifang, CHEN Jigang
Organization: Municipal Key Laboratory of Microorganism and Environmental Engineering, College of Biological and Environmental Sciences, Zhejiang Wanli University
Abstract: Seawater from B80-3.5 km, B79-100 m, C19-42 m three stations of Arctic ocean was brought by the Arctic expedition team conductivity temperature depth (CTD), bacteria were cultivated in the dialysis room continuously. 16S rDNA gene library was used to analyze the bacterial community diversity. The results indicated that the bacterial community diversity was high. The culturable bacteria of B80-3.5 km station could be divided into four groups: Alphaproteobacteria 66.66%, Gammaproteobacteria 21.21%, Cytophagia 4.04%, uncultured 8.08%. The culturable bacteria of B79-100 m station could be divided into four groups: Alphaproteobacteria 83.64%, Gammaproteobacteria 10.20%, Cytophagia 3.06%, uncultured 3.06%. The culturable bacteria of C19-42 m station could be divided into four groups: Alphaproteobacteria 69.69%, Gammaproteobacteria 12.12%, Cytophagia 5.05%, uncultured 13.13%.
Key words: marine microorganism; Arctic seawater; dialysis room enrichment culture; 16S rDNA library; marine bacterial diversity
发表期数: 2014年9月第17期
引用格式: 饶萌,杨季芳,陈吉刚. 北冰洋海水可培养微生物多样性分析[J]. 中国科技论文在线精品论文,2014,7(17):1709-1715.
 
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