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骨髓间充质干细胞共培养对大鼠肝癌细胞增殖的影响

发表时间:2014-09-15  浏览量:1407  下载量:588
全部作者: 郭庆,李晓明,宋关斌
作者单位: 昆明学院生命科学与技术系;重庆大学生物工程学院
摘 要: 目的:探讨肝癌细胞在间充质干细胞(mesenchymal stem cells,MSCs)共存条件下增殖行为的变化特点及相关分子机理。方法:原代分离培养大鼠骨髓MSCs,采用transwell系统进行MSCs和大鼠肝癌细胞CBRH-7919共培养,用MTT方法分析共培养条件下CBRH-7919细胞的增殖情况,用RT-PCR和Western blotting技术检测相关分子mRNA和蛋白水平的表达变化。结果:与MSCs共培养明显促进CBRH-7919细胞的增殖能力,并诱导CBRH-7919细胞Wnt5a, β-catenin基因的表达和β-catenin蛋白水平的表达。结论:MSCs分泌相关因子可能通过Wnt5a, β-catenin信号促进CBRH-7919细胞的增殖。
关 键 词: 细胞生物学;间充质干细胞;肝癌细胞;共培养;增殖;分子机理
Title: Effect of cocultured bone marrow mesenchymal stem cells on the proliferation of rat hepatoma cells
Author: GUO Qing, LI Xiaoming, SONG Guanbin
Organization: Department of Life Science and Technology, Kunming University; College of Bioengineering, Chongqing University
Abstract: Objective: To study the change of proliferation and correlated molecular mechanism of hepatoma cells under the condition of co-culture with mesenchymal stem cells (MSCs). Methods: using a transwell system, we co-cultured rat hepatoma cells (CBRH-7919) with rat bone marrow-derived MSCs, and then examined the effect of MSCs on proliferation of CBRH-7919 cells. MTT assay was used to check the proliferation, RT-PCR and Western blotting technology were used to check the mRNA and protein changes. Results: The co-culture with MSCs and showed that a significant increase of proliferation of CBRH-7919 cells, and induced the expression of Wnt5a, β-catening enes and β-catenin protein. Conclusion: MSCs could The results demonstrated that MSCs could promote CBRH-7919 cells proliferation, through Wnt5a/β-catenin pathways.
Key words: cell biology; mesenchymal stem cells; hepatoma cells; co-culture; proliferation; molecular mechanism
发表期数: 2014年9月第17期
引用格式: 郭庆,李晓明,宋关斌. 骨髓间充质干细胞共培养对大鼠肝癌细胞增殖的影响[J]. 中国科技论文在线精品论文,2014,7(17):1716-1722.
 
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