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DNA marker Ⅲ标准分子量参照物的2种制备方法

发表时间:2014-09-15  浏览量:1473  下载量:511
全部作者: 张煜隆,衣雨娇,刘小娟,洪彬湖,江l劊庾娼ǎ钗
作者单位: 福建农林大学植物保护学院;福建农林大学福建省植物病毒学重点实验室
摘 要: 目前,市售的DNA marker多使用酶切质粒或噬菌体DNA的方法进行制作,其设计和生产成本较高。研究提供了2种DNA marker Ⅲ的制作方法,一种是完全通过PCR的方法直接制备,另一种则结合了PCR和质粒酶切手段。实验结果证明:2种方法所制备的DNA marker Ⅲ均条带清晰,大小与市售产品完全吻合,并且成本大幅下降,其中通过PCR方法制备的DNA marker Ⅲ产量更大,成本更低,而将PCR方法与酶切质粒的方法相结合所制备的DNA marker Ⅲ条带更清晰。
关 键 词: 分子生物学;DNA marker;核酸电泳;PCR扩增;单酶切
Title: Two preparation methods of DNA marker Ⅲ for standard molecular weight reference
Author: ZHANG Yulong, YI Yujiao, LIU Xiaojuan, HONG Binhu, JIANG Yun, WU Zujian, LI Weimin
Organization: College of Plant Protection, Fujian Agriculture and Forestry University; Fujian Provincial Key Laboratory of Plant Virology, Fujian Agriculture and Forestry University
Abstract: At present, a lot of commercially available DNA markers are made of enzyme digested plasmid or phage, this method needs higher cost in production. This paper presents two methods to produce DNA marker Ⅲ. One is PCR method to produce DNA marker Ⅲ directly, and the other is a combination of PCR method and endonuclease digestion method. Experimental results show that with lower cost, the DNA marker Ⅲ bands of these two methods are both clear and the sizes are perfectly matched. Using PCR method to produce DNA marker Ⅲ provides a larger yield and a lower cost, yet bands are more clearly when DNA marker Ⅲ is produced by the combination method.
Key words: molecular biology; DNA marker; nucleic acid electrophoresis; PCR amplification; single endonuclease digestion
发表期数: 2014年9月第17期
引用格式: 张煜隆,衣雨娇,刘小娟,等. DNA marker Ⅲ标准分子量参照物的2种制备方法[J]. 中国科技论文在线精品论文,2014,7(17):1737-1741.
 
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