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活化-冻干壳聚糖微球固定脂肪酶的研究

发表时间:2014-09-15  浏览量:1422  下载量:421
全部作者: 刘君腾,赵晨希,陈旸,任钟旗,张卫东
作者单位: 北京化工大学化学工程学院
摘 要: 研究采用真空冷冻干燥技术制备了具有空间网状结构的壳聚糖微球,考察了冻干对载体形貌及活性位点的影响。以活化-冻干处理后的壳聚糖微球为载体固定假丝酵母脂肪酶(Lipase EC3.1.1.3),对固定化效果进行研究,并与直接固定和活化-固定的固定化酶进行对比,考察了活化过程对固定化酶性能的影响。结果表明,采用活化-冻干-固定法制备的固定化酶性能优良,其固定脂肪酶的水解活力可达57.2 IU/g.
关 键 词: 生物化学;固定化;活化-冻干;脂肪酶;壳聚糖
Title: Study on lipase immobilization of activated-freeze-drying chitosan microsphere
Author: LIU Junteng, ZHAO Chenxi, CHEN Yang, REN Zhongqi, ZHANG Weidong
Organization: College of Chemical Engineering, Beijing University of Chemical Technology
Abstract: In this paper, vacuum freeze-drying technology was used to prepare the chitosan microspheres, which had three-dimensional spatial network structure. The effects of vacuum freeze-drying on the morphology of microspheres and active sites were investigated. Candida lipase (Lipase EC3.1.1.3) were immobilized on the activated-lyophilized chitosan microspheres. The immobilization performance of these microspheres was studied and compared with direct-immobilization and activated-immobilization. The influence of activation process on the performance of immobilized lipase was also illustrated. The experimental results indicated that using activated-lyophilized chitosan microspheres as carrier to immobilize lipase is an optimal choice, since the activity of immobilized lipase was over 57.2 IU/g.
Key words: biochemistry; immobilization; activated-freeze-drying; lipase; chitosan
发表期数: 2014年9月第17期
引用格式: 刘君腾,赵晨希,陈旸,等. 活化-冻干壳聚糖微球固定脂肪酶的研究[J]. 中国科技论文在线精品论文,2014,7(17):1742-1746.
 
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