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LTB-SMS融合蛋白的构建、表达及免疫原性研究

发表时间:2014-09-15  浏览量:1433  下载量:428
全部作者: 谢瑞敏,李军,姚雪梅,齐兴柱
作者单位: 海南大学海洋学院;海南大学热带生物资源教育部重点实验室
摘 要: 研究克隆了产肠毒素性大肠杆菌(enterotoxigenic Escherichia coli, ETEC)不耐热肠毒素B亚单位(heat-labile enterotoxin B, LTB)基因,并用化学方法合成了生长抑素(somatostatin, SMS)基因,构建了LTB与SMS重组子,进行了重组子大肠杆菌原核表达融合蛋白(LTB-SMS);LTB-SMS纯化鉴定后在家兔体内进行了皮下和黏膜免疫研究。研究结果表明:LTB-SMS的血清抗体滴度达到较高水平,说明LTB可有效增强SMS的免疫原性。研究结果为进一步开发生长抑素基因工程疫苗奠定了良好的基础。
关 键 词: 生物工程;大肠杆菌不耐热肠毒素;生长抑素;融合蛋白;免疫原性
Title: Construction, expression and immunogenicity research of LTB-SMS fusion protein
Author: XIE Ruimin, LI Jun, YAO Xuemei, QI Xingzhu
Organization: Ocean College, Hainan University; Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan University
Abstract: Heat-labile enterotoxin B (LTB) gene was first cloned from enterotoxigenic Escherichia coli (ETEC), and somatostatin (SMS) gene was chemically synthesized. The recombinant of LTB and SMS was constructed, and fusion protein (LTB-SMS) was expressed in E.coli. The immunological characteristics of LTB-SMS purified and determined were studied in rabbits by subcutaneous and mucosal immunization. The results showed that the serum antibody titers of LTB-SMS reached high level, which indicated that LTB can effectively enhance the immunogenicity of SMS. This research laid a good foundation for the further development of somatostatin genetic engineering vaccine.
Key words: bioengineering; E.coli heatlabileenterotoxin; somatostatin; fusion protein; immunogenicity
发表期数: 2014年9月第17期
引用格式: 谢瑞敏,李军,姚雪梅,等. LTB-SMS融合蛋白的构建、表达及免疫原性研究[J]. 中国科技论文在线精品论文,2014,7(17):1747-1751.
 
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