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利用DNA条形码技术鉴定北京延庆松山地区舟蛾科方法的研究

发表时间:2014-09-15  浏览量:1409  下载量:485
全部作者: 于晓溪,周晓雪,杨聪慧,武春生,张爱兵
作者单位: 首都师范大学生命科学学院;中国科学院动物研究所动物进化与系统学院重点实验室
摘 要: 目的:采用DNA条形码技术鉴定北京延庆松山地区的鳞翅目(Lepidoptera)舟蛾科(Notodontidae)昆虫。方法:提取蛾类样本DNA,对线粒体细胞色素C氧化酶亚基I(mitochondrial cytochrome C oxidase subunitⅠ, COI)基因进行扩增和测序。分别利用建立系统进化树、诊断特征位点及通用混合Yule溯祖模型(general mixed Yule-coalescent, GMYC)的方法对舟蛾科进行聚类。结果与结论:这3种方法均能较好地将其区分归类。
关 键 词: 生态学;舟蛾科;DNA条形码;系统发育;诊断特征位点;通用混合Yule溯祖模型
Title: Identification of moth species (Notodontidae) from Songshan, Yanqing in Beijing using DNA barcoding
Author: YU Xiaoxi, ZHOU Xiaoxue, YANG Conghui, WU Chunsheng, ZHANG Aibing
Organization: School of Life Sciences, Capital Normal University; Key Laboratory of Zoological Systematics and Evolution, Institute of Zoology, Chinese Academy of Sciences
Abstract: Objective: To identify moth species (Notodontidae) from Songshan, Yanqing in Beijing using DNA barcoding. Methods: Total genomic DNA was extracted from specimens of moths. Mitochondrial mitochondrial cytochrome C oxidase subunitⅠ (COI) sequences were amplified. Species identifications were performed using system evolutionary, diagnostic characters and general mixed Yule-coalescent (GMYC) methods. Results and conclusion: DNA barcoding produces good classification results and all these methods could classify these species correctly.
Key words: ecology; Notodontidae; DNA barcoding; phylogenetic tree; diagnostic character site; general mixed Yule-coalescent
发表期数: 2014年9月第17期
引用格式: 于晓溪,周晓雪,杨聪慧,等. 利用DNA条形码技术鉴定北京延庆松山地区舟蛾科方法的研究[J]. 中国科技论文在线精品论文,2014,7(17):1764-1771.
 
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