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悬铃木LFY同源基因启动子的功能分析

发表时间:2014-10-15  浏览量:1996  下载量:659
全部作者: 陆顺教,易双双,李志能,刘蕾,张佳琪,包满珠,刘国锋
作者单位: 华中农业大学园艺林学学院,园艺植物生物学教育部重点实验室;中国热带农业科学院热带作物品种资源研究所,农业部华南作物基因资源与种质创制重点开放实验室;西南大学园艺园林学院;西安铁路北客站地区管理委员会
摘 要: LEAFY(LFY)基因是植物花分生组织决定基因,对植物花芽分化及花发育具有重要调控作用。为探讨悬铃木LFY同源基因启动子(pPlacLFY)用于创造不育转基因植株的可行性,通过构建pPlacLFY::GUS 和pPlacLFY::BARNASE载体转化烟草,对其表达特性和功能进行研究。对转pPlacLFY::GUS 烟草T1代植株不同部位的组织化学染色及GUS蛋白定量分析结果显示:在花萼、花瓣、雄蕊、雌蕊、果实等生殖器官中均有很强的GUS活性,其中果实的表达量最高;在幼叶、茎和顶芽等营养器官中也检测到较高的GUS活性,而在老叶和根中GUS表达不明显。对pPlacLFY::BARNASE转基因烟草的表型观测结果发现:多数转化植株的生长发育受到影响,主要表现为生长缓慢、叶片扭曲皱缩、出现黄斑和顶芽萎缩等,最后逐渐死亡。由此可见,悬铃木的LFY同源基因具有较广泛的表达特性,不适合直接用于基因工程创造不育植株,同时也说明PlacLFY基因在参与生殖发育调控的同时,还可能参与营养器官的发育。
关 键 词: 园林植物学;悬铃木;LEAFY;启动子;BARNASE;不育
Title: Functional characterization of LFY homologous gene promoter from London plane (Platanus acerifolia Willd.)
Author: LU Shunjiao, YI Shuangshuang, LI Zhineng, LIU Lei, ZHANG Jiaqi, BAO Manzhu, LIU Guofeng
Organization: Key Laboratory of Horticultural Plant Biology, Ministry of Education, College of Horticulture and Forestry Sciences, Huazhong Agricultural University; Key Laboratory of Crop Gene Resources and Germplasm Enhancement in Southern China, Tropical Crops Genetic Resources Institute, Chinese Academy of Tropical Agricultural Sciences; College of Horticulture and Landscape Architecture, Southwest University; District Management Committee of Xi’an North Railway Station
Abstract: LEAFY (LFY) is the floral meristem identity gene that plays a key role in flower bud differentiation and flower development. To investigate the feasibility of using London plane LFY homologous gene promoter (pPlacLFY) to create transgenic sterile plants, two expression plasmid vectors pPlacLFY::GUS and pPlacLFY::BARNASE were constructed and then transformed into tobacco plants. Histochemical staining and protein quantitation assays for GUS (beta-glucoronidase) activity of T1 transgenic tobacco plants with pPlacLFY::GUS were investigated, which indicated that the GUS gene were highly expressed in all reproductive organs of transgenic plants including the sepals, petals, stamens, carpels and fruits, with the strongest expression in the fruits. GUS expression was also evidently detected in young leaves, stems and shoot tips, but not in old leaves and roots. Transgenic tobacco plants with pPlacLFY::BARNASE frequently showed inhibited growth and development, such as growth retardation, curled leaves, yellowing leaves, or shriveled stem tips. Most of them finally died before up to flowering. These results suggest that the LFY homologous gene of London plane is not only expressed highly in reproductive organs but also in vegetative tissues, so its promoter will not suitable for using directly in genetic engineering of sterility. The results also imply that the PlacLFY gene may play some important roles in vegetative organ development except for their functions during flower initiation and development.
Key words: garden botany; Platanus acerifolia; LEAFY; promoter; BARNASE; steriltiy
发表期数: 2014年10月第19期
引用格式: 陆顺教,易双双,李志能,等. 悬铃木LFY同源基因启动子的功能分析[J]. 中国科技论文在线精品论文,2014,7(19):1913-1920.
 
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