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高效液相色谱串联质谱法检测鱼肉组织中的羟基多溴联苯醚
发表时间：2014-10-15 浏览量：1980 下载量：552
| 全部作者： | 刘欣，姚永青，李培，陈英，丁洪流，纪丽君，姚卫蓉 |
| 作者单位： | 江南大学食品学院；苏州市产品质量监督检验所 |
| 摘 要： | 研究建立了检测鱼肉组织中7种羟基多溴联苯醚（hydroxylated polybrominated diphenyl ethers, PBDEs）即OH-PBDEs的方法。鱼样品经过正己烷/丙酮（体积比为1：1）混合液超声波辅助提取（ultrasonic-assisted extraction, UAE），固相萃取（solid-phase extraction, SPE）净化后，采用液相色谱-电喷雾电离串联质谱法（liquid chromatography-electrospray ionization-tandem quadrupole mass spectrometry, LC-ESI-MS/MS）在负离子模式下检测，外标法定量。结果表明：7种OH-PBDEs的回收率为71.11%~99.30%，相对标准偏差（relative standard deviation, RSD）在3.35%~18.41%之间，方法检测限（method detection limits, MDLs）为0.10~0.25 ng/g（干重）. 利用该方法对8种鱼进行OH-PBDEs检测，结果显示：7种物质均有检出，其中6-OH-BDE-47和2’-OH-BDE-68的含量较高，最高分别可达3.00 ng/g（干重）和1.85 ng/g（干重）. |
| 关 键 词： | 食品检验学；羟基多溴联苯醚；液相色谱-电喷雾电离串联质谱；鱼肉组织 |
| Title： | Detection of hydroxylated polybrominated diphenyl ethers in fish tissue by using ultra performance liquid chromatography-tandem mass spectrometry |
| Author： | LIU Xin, YAO Yongqing, LI Pei, CHEN Ying, DING Hongliu, JI Lijun, YAO Weirong |
| Organization： | School of Food Science & Technology, Jiangnan University; Suzhou Product Quality Supervision and Inspection Institute |
| Abstract： | A reliable analytical method was developed here for seven hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in fish. Ultrasonic-assisted extraction(UAE)was employed with n-hexane/acetone(the volume ratio was 1：1), and solid-phase extraction(SPE)were used for cleaning-up the extract of fish. The final extract was identified and quantified by liquid chromatography coupled with liquid chromatography-electrospray ionization-tandem quadrupole mass spectrometry (LC-ESI-MS/MS) with external standard method. The recoveries of the spiked standards ranged from 71.11％ to 99.30％, and the relative standard deviation (RSD) was 3.35% to 18.41%, the method detection limits (MDLs) ranged from 0.10 to 0.25 ng/g (dry weight). Eight kinds of fish were detected with this method for OH-PBDEs. The results showed that seven substances were all detected, of which 6-OH-BDE-47 and 2’-OH-BDE-68 were higher levels, 3.00 ng/g (dry weight) and 1.85 ng/g (dry weight), respectively. |
| Key words： | food inspection; OH-hydroxylated polybrominated diphenyl ethers; liquid chromatography-electrospray ionization-tandem quadrupole mass spectrometry; fish tissue |
| 发表期数： | 2014年10月第19期 |
| 引用格式： | 刘欣，姚永青，李培，等. 高效液相色谱串联质谱法检测鱼肉组织中的羟基多溴联苯醚[J]. 中国科技论文在线精品论文，2014，7（19）：1978-1985. |
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