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高效液相色谱串联质谱法检测鱼肉组织中的羟基多溴联苯醚

发表时间:2014-10-15  浏览量:1725  下载量:511
全部作者: 刘欣,姚永青,李培,陈英,丁洪流,纪丽君,姚卫蓉
作者单位: 江南大学食品学院;苏州市产品质量监督检验所
摘 要: 研究建立了检测鱼肉组织中7种羟基多溴联苯醚(hydroxylated polybrominated diphenyl ethers, PBDEs)即OH-PBDEs的方法。鱼样品经过正己烷/丙酮(体积比为1:1)混合液超声波辅助提取(ultrasonic-assisted extraction, UAE),固相萃取(solid-phase extraction, SPE)净化后,采用液相色谱-电喷雾电离串联质谱法(liquid chromatography-electrospray ionization-tandem quadrupole mass spectrometry, LC-ESI-MS/MS)在负离子模式下检测,外标法定量。结果表明:7种OH-PBDEs的回收率为71.11%~99.30%,相对标准偏差(relative standard deviation, RSD)在3.35%~18.41%之间,方法检测限(method detection limits, MDLs)为0.10~0.25 ng/g(干重). 利用该方法对8种鱼进行OH-PBDEs检测,结果显示:7种物质均有检出,其中6-OH-BDE-47和2’-OH-BDE-68的含量较高,最高分别可达3.00 ng/g(干重)和1.85 ng/g(干重).
关 键 词: 食品检验学;羟基多溴联苯醚;液相色谱-电喷雾电离串联质谱;鱼肉组织
Title: Detection of hydroxylated polybrominated diphenyl ethers in fish tissue by using ultra performance liquid chromatography-tandem mass spectrometry
Author: LIU Xin, YAO Yongqing, LI Pei, CHEN Ying, DING Hongliu, JI Lijun, YAO Weirong
Organization: School of Food Science & Technology, Jiangnan University; Suzhou Product Quality Supervision and Inspection Institute
Abstract: A reliable analytical method was developed here for seven hydroxylated polybrominated diphenyl ethers (OH-PBDEs) in fish. Ultrasonic-assisted extraction(UAE)was employed with n-hexane/acetone(the volume ratio was 1:1), and solid-phase extraction(SPE)were used for cleaning-up the extract of fish. The final extract was identified and quantified by liquid chromatography coupled with liquid chromatography-electrospray ionization-tandem quadrupole mass spectrometry (LC-ESI-MS/MS) with external standard method. The recoveries of the spiked standards ranged from 71.11% to 99.30%, and the relative standard deviation (RSD) was 3.35% to 18.41%, the method detection limits (MDLs) ranged from 0.10 to 0.25 ng/g (dry weight). Eight kinds of fish were detected with this method for OH-PBDEs. The results showed that seven substances were all detected, of which 6-OH-BDE-47 and 2’-OH-BDE-68 were higher levels, 3.00 ng/g (dry weight) and 1.85 ng/g (dry weight), respectively.
Key words: food inspection; OH-hydroxylated polybrominated diphenyl ethers; liquid chromatography-electrospray ionization-tandem quadrupole mass spectrometry; fish tissue
发表期数: 2014年10月第19期
引用格式: 刘欣,姚永青,李培,等. 高效液相色谱串联质谱法检测鱼肉组织中的羟基多溴联苯醚[J]. 中国科技论文在线精品论文,2014,7(19):1978-1985.
 
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