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重金属铜对克氏原螯虾细胞免疫功能的影响

发表时间:2014-11-15  浏览量:1932  下载量:482
全部作者: 庞胜喜,魏克强,赵婷,王靖
作者单位: 山西省土地整理中心;山西大学生命科学学院
摘 要: 研究水体中不同浓度的Cu2+对克氏原螯虾(Procambarus clarkii)细胞免疫功能的影响,分别采用0,0.5,1.0,3.0,5.0,10.0 mg/L Cu2+刺激克氏原螯虾,于24,48,72,96 h后测定血淋巴中的血细胞总数(total number of blood cells, THC)和吞噬活性。结果表明,各处理组的THC均低于对照组,但血细胞的形态没有明显变化;与对照组比较,1.0,3.0,5.0,10.0 mg/L组血细胞的吞噬百分比和吞噬指数呈现显著下降的趋势(P≤0.05)。结果提示,水环境中重金属Cu2+对克氏原螯虾的细胞免疫功能具有显著影响,对机体抵抗力构成了潜在威胁。
关 键 词: 水产生物学;环境暴露;免疫响应;克氏原螯虾;吞噬作用
Title: Effects of copper on the cell immunity of Procambarus clarkii
Author: PANG Shengxi, WEI Keqiang, ZHAO Ting, WANG Jing
Organization: Shanxi Center of Land Consolidation and Rehabilitation; School of Life Science, Shanxi University
Abstract: In order to study the effects of Cu2+ on cell immunity of the crayfish Procambarus clarkii, crayfish were exposed to different concentrations of Cu2+ at 0, 0.5, 1.0, 3.0, 5.0, 10.0 mg /L. The total number of blood cells (THC) and phagocytic activity were determined after exposure at 24, 48, 72, 96 h, respectively. The results showed that THC in each group was lower than that in the control group. The blood cell morphology did not change significantly. When crayfish were exposed to the concentrations at 1.0, 3.0, 5.0, 10.0 mg/L Cu2+, the phagocytic percent and phagocytic index were lower than that in the control group (P≤0.05). The results suggested that Cu2+ had a significant negative effect on cell immunity in P.clarkii, which indicated that heavy metal pollution had potential threat to the health of crayfish.
Key words: aquatic biology; environmental exposure; immune response; Procambarus clarkii; phagocytosis
发表期数: 2014年11月第21期
引用格式: 庞胜喜,魏克强,赵婷,等. 重金属铜对克氏原螯虾细胞免疫功能的影响[J]. 中国科技论文在线精品论文,2014,7(21):2190-2194.
 
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