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细辛附子不同剂量配比对心肾阳虚缓慢性心律失常大鼠血清Na+-K+-ATPase及cAMP/cGMP的影响
发表时间：2014-12-15 浏览量：2097 下载量：520
| 全部作者： | 张明飞，刘兴隆，贾波，沈涛，由凤鸣，张晓丹，姜岑 |
| 作者单位： | 南康区第一人民医院内科；成都中医药大学基础医学院 |
| 摘 要： | 目的：考察细辛附子不同配比对心肾阳虚型缓慢性心律失常（Brady arrhythmia，Ba）大鼠心率和血清Na+-K+-ATPase及环磷酸鸟苷（cyclic guanine monophosphate, cGMP)与环磷酸腺苷（cyclic adenosine monophosphate，cAMP）的影响，探讨大鼠体内离子通道异常与Ba的相关性及细辛附子不同配比对其干预作用。方法：采用阿霉素加氢化可的松腹腔注射的方法复制心肾阳虚型大鼠模型，以细辛附子不同剂量配比煎液灌胃给药，酶联免疫吸附法(enzyme linked immuno sorbent assay，ELISA)检测血清Na+-K+-ATPase，cGMP及cAMP的含量。结果：与空白组相比，模型组Na+-K+-ATPase，cAMP含量降低，cGMP含量升高。经药物干预后，Ⅳ组剂量配比（细辛∶附子=6∶12）可显著提高大鼠Na+-K+-ATPase，cAMP含量，降低cGMP含量。结论：细辛附子配伍能纠正大鼠体内离子通道异常，改善心肌细胞自律性,发挥治疗Ba的作用，尤以Ⅳ组(细辛∶附子=6∶12)剂量配比组作用最佳。 |
| 关 键 词： | 中药学；细辛附子；心肾阳虚缓慢性心律失常；Na+-K+-ATPase；环磷酸鸟苷；环磷酸腺苷 |
| Title： | Effect of Xixin and Fuzi on serum Na+-K+-ATPase and cAMP/cGMP with different proportions of Brady arrhythmia model rat with heart-kidney yang deficiency |
| Author： | ZHANG Mingfei, LIU Xinglong, JIA Bo, SHEN Tao, YOU Fengming, ZHANG Xiaodan, JIANG Cen |
| Organization： | Department of Internal Medicine,The First People’s Hospital of Nankang; College of Basic Medicine, Chengdu University of Traditional Chinese Medicine |
| Abstract： | Objective: To observe the effect of Xixin and Fuzi on Na+-K+-ATPase, cyclic guanine monophosphate (cGMP) and cyclic guanine monophosphate (cAMP) with different proportions on Brady arrhythmia（Ba）model rat with heart-kidney yang deficiency，and study the relationship of abnormal ion channels with Ba, and the intervene of Xixin with Fuzi on it. Methods: In this study, we used heart-kidney yang deficiency molding method together with intraperitoneal injection of adriamycin and hydrocortisone to Brady arrhythmia（Ba） rat model with heart-kidney yang deficiency. The model rats were treated by Xixin and Fuzi with different proportions, and the content of serum Na+-K+-ATPase， cGMP and cAMP were investigated by using enzyme linked immuno sorbent assay (ELISA). Results: Compared with blank control group, the serum content of cGMP increased significantly, but serum content of Na+-K+-ATPase, cAMP decreased significantly. After the intervene with Xixin and Fuzi, the group of Ⅳ(Xixin∶Fuzi=6∶12) could up-regulate the level of Na+-K+-ATPase and cAMP, and had obvious effect of down-regulating the level of cGMP. Conclusion: Xixin and Fuzi decoction can correct body ion channels in rat anomaly, improve myocardial cell contraction and play a role on curing Ba. Moreover, the effect of Ⅳ (Xixin∶Fuzi=6∶12) is the best. |
| Key words： | traditional Chinese pharmacology; Xixin and Fuzi; Brady arrhythmia model with heart-kidney yang deficiency; Na+-K+-ATPase; cyclic guanine monophosphate; cyclic adenosine monophosphate |
| 发表期数： | 2014年12月第23期 |
| 引用格式： | 张明飞，刘兴隆，贾波，等. 细辛附子不同剂量配比对心肾阳虚缓慢性心律失常大鼠血清Na+-K+-ATPase及cAMP/cGMP的影响[J]. 中国科技论文在线精品论文，2014，7（23）：2317-2322. |
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