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细辛附子不同剂量配比对心肾阳虚缓慢性心律失常大鼠血清Na+-K+-ATPase及cAMP/cGMP的影响

发表时间:2014-12-15  浏览量:1547  下载量:439
全部作者: 张明飞,刘兴隆,贾波,沈涛,由凤鸣,张晓丹,姜岑
作者单位: 南康区第一人民医院内科;成都中医药大学基础医学院
摘 要: 目的:考察细辛附子不同配比对心肾阳虚型缓慢性心律失常(Brady arrhythmia,Ba)大鼠心率和血清Na+-K+-ATPase及环磷酸鸟苷(cyclic guanine monophosphate, cGMP)与环磷酸腺苷(cyclic adenosine monophosphate,cAMP)的影响,探讨大鼠体内离子通道异常与Ba的相关性及细辛附子不同配比对其干预作用。方法:采用阿霉素加氢化可的松腹腔注射的方法复制心肾阳虚型大鼠模型,以细辛附子不同剂量配比煎液灌胃给药,酶联免疫吸附法(enzyme linked immuno sorbent assay,ELISA)检测血清Na+-K+-ATPase,cGMP及cAMP的含量。结果:与空白组相比,模型组Na+-K+-ATPase,cAMP含量降低,cGMP含量升高。经药物干预后,Ⅳ组剂量配比(细辛∶附子=6∶12)可显著提高大鼠Na+-K+-ATPase,cAMP含量,降低cGMP含量。结论:细辛附子配伍能纠正大鼠体内离子通道异常,改善心肌细胞自律性,发挥治疗Ba的作用,尤以Ⅳ组(细辛∶附子=6∶12)剂量配比组作用最佳。
关 键 词: 中药学;细辛附子;心肾阳虚缓慢性心律失常;Na+-K+-ATPase;环磷酸鸟苷;环磷酸腺苷
Title: Effect of Xixin and Fuzi on serum Na+-K+-ATPase and cAMP/cGMP with different proportions of Brady arrhythmia model rat with heart-kidney yang deficiency
Author: ZHANG Mingfei, LIU Xinglong, JIA Bo, SHEN Tao, YOU Fengming, ZHANG Xiaodan, JIANG Cen
Organization: Department of Internal Medicine,The First People’s Hospital of Nankang; College of Basic Medicine, Chengdu University of Traditional Chinese Medicine
Abstract: Objective: To observe the effect of Xixin and Fuzi on Na+-K+-ATPase, cyclic guanine monophosphate (cGMP) and cyclic guanine monophosphate (cAMP) with different proportions on Brady arrhythmia(Ba)model rat with heart-kidney yang deficiency,and study the relationship of abnormal ion channels with Ba, and the intervene of Xixin with Fuzi on it. Methods: In this study, we used heart-kidney yang deficiency molding method together with intraperitoneal injection of adriamycin and hydrocortisone to Brady arrhythmia(Ba) rat model with heart-kidney yang deficiency. The model rats were treated by Xixin and Fuzi with different proportions, and the content of serum Na+-K+-ATPase, cGMP and cAMP were investigated by using enzyme linked immuno sorbent assay (ELISA). Results: Compared with blank control group, the serum content of cGMP increased significantly, but serum content of Na+-K+-ATPase, cAMP decreased significantly. After the intervene with Xixin and Fuzi, the group of Ⅳ(Xixin∶Fuzi=6∶12) could up-regulate the level of Na+-K+-ATPase and cAMP, and had obvious effect of down-regulating the level of cGMP. Conclusion: Xixin and Fuzi decoction can correct body ion channels in rat anomaly, improve myocardial cell contraction and play a role on curing Ba. Moreover, the effect of Ⅳ (Xixin∶Fuzi=6∶12) is the best.
Key words: traditional Chinese pharmacology; Xixin and Fuzi; Brady arrhythmia model with heart-kidney yang deficiency; Na+-K+-ATPase; cyclic guanine monophosphate; cyclic adenosine monophosphate
发表期数: 2014年12月第23期
引用格式: 张明飞,刘兴隆,贾波,等. 细辛附子不同剂量配比对心肾阳虚缓慢性心律失常大鼠血清Na+-K+-ATPase及cAMP/cGMP的影响[J]. 中国科技论文在线精品论文,2014,7(23):2317-2322.
 
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