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响应面分析法优化蔷薇红景天多酚提取工艺

发表时间:2014-12-15  浏览量:1400  下载量:547
全部作者: 赵岩,赵天琦,蔡恩博,许永华,刘双利,杨鹤,张连学
作者单位: 吉林农业大学中药材学院
摘 要: 目的:研究蔷薇红景天多酚的最佳提取工艺条件。方法:利用响应面法(response surface method,RSM)对蔷薇红景天多酚的提取工艺进行优化。在单因素实验的基础上,选择乙醇浓度、超声提取时间和液固比为自变量,多酚提取率为响应值,根据中心组合实验设计原理采用3因素5水平的响应面分析法,研究各自变量及其交互作用对多酚提取率的影响,模拟得到二次多项式回归方程的预测模型,并对最优条件进行验证。结果:在分析各因素的显著性和交互作用后,得出蔷薇红景天多酚的最佳提取工艺为:乙醇浓度48%(体积比),超声时间40 min,液固比286 mL/g. 结论:在最佳工艺条件下,蔷薇红景天多酚提取率为11.59%(质量比). 研究建立的模型适合蔷薇红景天多酚提取,可用于实际生产。
关 键 词: 中药化学;蔷薇红景天;多酚;响应面法
Title: Ultrasonic extraction technology optimization of polyphenols from Rhodiola rosea by response surface methodology
Author: ZHAO Yan, ZHAO Tianqi, CAI Enbo, XU Yonghua, LIU Shuangli, YANG He, ZHANG Lianxue
Organization: College of Chinese Medicinal Materials, Jilin Agricultural University
Abstract: Objective: The paper studies extraction technique conditions of polyphenols from Rhodiola rosea. Methods: Using the response surface method (RSM), we optimize the extraction process of polyphenols from R.rosea. On the basis of single factor experiments, the ethanol concentration, ultrasonic extraction time and liquid-solid ratio are used as independent variables, polyphenols extraction rate as response value. According to the principle of center combination experiment designed with 3 factors and 5 levels of response surface analysis, interaction of each variable and its influence on the yield of polyphenols, simulation prediction model of quadratic polynomial regression equation was obtained. Results: After analyzing the significant interaction and the various factors, the optimum polyphenol extraction technology are as following: ethanol concentration 48% (volume ratio), ultrasonic extraction time 40 min, liquid-solid ratio 286 mL/g. Conclusion: Under the condition of optimum process, the polyphenols yield is 11.59%. The research model is suitable for polyphenols extraction from R.rosea, which can be used in practical production.
Key words: Chinese medicine chemistry; Rhodiola rosea; polyphenols; response surface method
发表期数: 2014年12月第23期
引用格式: 赵岩,赵天琦,蔡恩博,等. 响应面分析法优化蔷薇红景天多酚提取工艺[J]. 中国科技论文在线精品论文,2014,7(23):2323-2330.
 
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