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左归丸对PMOP大鼠骨组织中TGF-β1/Smad4 mRNA表达的影响

发表时间:2014-12-15  浏览量:1292  下载量:396
全部作者: 任艳玲,吕海波,刘立萍,李娅玲
作者单位: 辽宁中医药大学基础医学院;鹤壁职业技术学院医学院
摘 要: 目的:研究左归丸对去卵巢骨质疏松大鼠骨组织中TGF-β1/Smad4 mRNA表达的影响,探讨其对骨质疏松症的防治机制。方法:SD雌性大鼠分为正常组,假手术组,模型空白组(ovariectomized,OVX),左归丸高、中、低剂量组,以及尼尔雌醇组,采用去卵巢的方法建立绝经后骨质疏松症模型,术后21 d开始给药:正常组、假手术组灌服生理盐水;左归丸高、中、低剂量组分别为6.4,3.2,1.6 g/kg,1次/d;尼尔雌醇组灌服0.021 g/kg,1次/周,连续120 d. 采用双能X线骨密度(bone mineral density,BMD)仪测定右后肢离体股骨近端1/3的BMD;采用逆转录-PCR(reverse transcription PCR, RT-PCR)法检测左后肢股骨近端1/3部分的TGF-β1/Smad4 mRNA表达水平。结果:与假手术组相比,模型组大鼠离体股骨近端BMD显著下降(P<0.01);与模型组相比,左归丸高、中、低剂量组及尼尔雌醇组BMD显著升高(P<0.01)。与假手术组相比,模型组大鼠骨组织中TGF-β1,Smad4 mRNA水平明显降低(P<0.01);左归丸各剂量组,骨组织中TGF-β1,Smad4 mRNA水平显著上升(P<0.01),各用药组之间无显著性差异(P>0.05)。结论:骨组织中TGF-β1,Smad4 mRNA表达水平的下调可能是绝经后骨质疏松症(postmenopausal osteoporosis,PMOP)发生的重要机制之一,左归丸能够上调骨组织中TGF-β1,Smad4 mRNA的表达,从而有效防治骨质疏松症。
关 键 词: 中医学;左归丸;绝经后骨质疏松症;TGF-β1;Smad4
Title: Effect of Zuogui pill on expressions of TGF-β1/Smad4 mRNA in bone tissues of PMOP rats
Author: REN Yanling, LÜ Haibo, LIU Liping, LI Yaling
Organization: School of Basic Medicine, Liaoning University of Traditional Chinese Medicine; Medical College, Hebi Polytechnic
Abstract: Objective: To study the effect of Zuogui pill on the expression of TGF-β1/Smad4 mRNA in bones and the mechanisms of preventing and curing osteoporosis. Methods: Female SD rats were distributed into normal group, sham-operation group, ovariectomized (OVX) group, high, medium and low dose of Zuogui pill groups, and nilestriol group. Rats of overiectomy-induced osteoporosis model were adopted. Rats were orally administrated physiological saline (negative group, sham group, OVX group), Zuogui pill (6.4, 3.2, 1.6 g/kg) once a day and nilestriol (0.021 g/kg, positive group) once a week for 120 d. The right hind proximate femur at the 1/3 side were determined the bone mineral density (BMD) by dual-energy X-ray BMD absorptiometry. TGF-β1 and Smad4 mRNA expression of the left hind proximate femur at the 1/3 side were evaluated by the method of reverse transcriptase-PCR (RT-PCR). Results: As compared with the sham group, OVX group markedly decreased BMD (P<0.01). Zuogui pill (6.4, 3.2, 1.6 g/kg) and nilestriol group markedly promoted BMD compared with the model group (P<0.01). As compared with the sham group, OVX group markedly reduced TGF-β1 and Smad4 mRNA expression (P<0.01). Zuogui pill (6.4, 3.2, 1.6 g/kg) induced expression of TGF-β1 and Smad4 mRNA in bone tissue (P0.05). Conclusion: TGF-β1 and Smad4 mRNA expression were downregulated in bone tissue of model rats. Zuogui pill can prevent and cure postmenopausal osteoporosis (PMOP) effectively, one of the mechanisms may be related to its up-regulating the expression of TGF-β1/Smad4 mRNA in bone tissue.
Key words: traditional Chinese medicine; Zuogui pill; postmenopausal osteoporosis; TGF-β1; Smad4
发表期数: 2014年12月第23期
引用格式: 任艳玲,吕海波,刘立萍,等. 左归丸对PMOP大鼠骨组织中TGF-β1/Smad4 mRNA表达的影响[J]. 中国科技论文在线精品论文,2014,7(23):2331-2336.
 
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