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Torin1及CQ对沙门菌-斑马鱼模型异源自噬的影响

发表时间:2014-12-15  浏览量:1500  下载量:422
全部作者: 李金玲,吴淑燕,李嫄渊,黄瑞
作者单位: 苏州大学基础医学与生物科学学院
摘 要: 目的:探究自噬激活剂Torin1及自噬抑制剂氯喹(chloroeuine,CQ)对鼠伤寒沙门菌引起的幼年斑马鱼体内异源自噬的影响。方法:用鼠伤寒沙门菌浸染受精后72 h的幼年斑马鱼。细菌生长曲线、斑马鱼存活率曲线及Western blotting法确定Torin1和CQ作用斑马鱼的适宜浓度;细菌计数法及Western blotting法探究二者对斑马鱼体内异源自噬的影响。结果:0.4 μmol/L Torin1作用幼年斑马鱼24 h后自噬标记蛋白Lc3-Ⅱ表达水平显著升高,自噬底物蛋白p62表达显著降低;50 μmol/L CQ作用幼鱼24 h后,Lc3-Ⅱ和p62表达水平均与上述相反。结论:Torin1和CQ对沙门菌感染斑马鱼引起的异源自噬的影响与二者对哺乳动物细胞自噬的影响一致。
关 键 词: 医学微生物;鼠伤寒沙门菌;斑马鱼;自噬
Title: Effects of Torin1 and CQ on xenophagy in Salmonella-zebrafish model
Author: LI Jinling, WU Shuyan, LI Yuanyuan, HUANG Rui
Organization: School of Biology & Basic Medical Sciences, Soochow University
Abstract: Objective: To explore the influence of activation agent Torin1 and autophagy inhibitor chloroeuine (CQ) on xenophagy of zebrafish larvae infected by Salmonella typhimurium of autophagy. Methods: Zebrafish larvae at 72 h post-fertilization were exposed to different doses of S.typhimurium. Bacterial growth curves, zebrafish survival curves and Western blotting were used to determine the suitable concentration of Torin1 and CQ. Bacteria enumberation and Western blotting were used to explore their impact on xenophagy of zebrafish larvae. Results: The larvae were treated with 0.4 μmol/L Torin1 for 24 h, the expression of autophagy protein Lc3-Ⅱwas significantly increased, but p62 was reduced. Lc3-Ⅱ and p62 expression levels of the larvae treated with 50 μmol/L CQ for 24 h were contrary to the above. Conclusion: Torin1 and CQ have the same influence on xenophagy of zebrafish larvae infected by S.typhimurium as it has on autophagy in mammalian cells.
Key words: medical microbiology; Salmonella typhimurium; zebrafish; autophagy
发表期数: 2014年12月第23期
引用格式: 李金玲,吴淑燕,李嫄渊,等. Torin1及CQ对沙门菌-斑马鱼模型异源自噬的影响[J]. 中国科技论文在线精品论文,2014,7(23):2355-2361.
 
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