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大气PM2.5诱发支气管的高反应性
发表时间：2014-12-15 浏览量：29904 下载量：14289
| 全部作者： | 王荣，肖雪，曹蕾，沈振兴，曹永孝 |
| 作者单位： | 西安交通大学医学部；西安交通大学能源与动力工程学院 |
| 摘 要： | 目的：探讨大气细颗粒物（fine particulate matter, PM2.5）的二甲基亚砜（DMSO）溶解物对大鼠支气管收缩功能的影响，并揭示其作用机制。方法：分离大鼠二级支气管，制成约1 mm长的环段，进行离体器官培养，培养后的支气管环采用小血管张力记录的方法，在组织浴槽中通过累加浓度给药法分别加入M胆碱受体激动剂卡巴胆碱，建立相应的收缩时效和量效曲线。结果：卡巴胆碱能浓度依赖性地收缩新鲜的大鼠支气管。与新鲜组相比，支气管在单纯培养12 h和24 h后，卡巴胆碱诱导的支气管收缩反应的最大收缩（the maximal contraction，Emax)和达到50%最大收缩时激动剂浓度的负对数(the negative logarithm of the concentration that produces 50% of the maximal contractile effect, pEC50)均无显著性差异。而培养48 h后，卡巴胆碱收缩支气管的量效曲线明显左移(P<0.01）。与DMSO溶剂对照组相比，PM2.5培养12，24，48 h均能增强卡巴胆碱引起的收缩反应，且培养24 h达到最大收缩反应；支气管环在0.3，1.0，3.0 mg/L PM2.5中培养24 h，均能增强卡巴胆碱引起的收缩反应，各组收缩反应的Emax分别为（149.4±9.9）%，（195.4±10.0）%和（143.9±7.7）%. 结论：PM2.5增强了M受体介导的支气管收缩作用，从而诱发大鼠支气管的高反应性。 |
| 关 键 词： | 药理学；气道高反应性；肌张力描记系统；细颗粒物 |
| Title： | Bronchus hyperresponsiveness induced by atmospheric PM2.5 |
| Author： | WANG Rong, XIAO Xue, CAO Lei, SHEN Zhenxing, CAO Yongxiao |
| Organization： | School of Medicine, Xi’an Jiaotong University; School of Energy and Power Engineering, Xi’an Jiaotong University |
| Abstract： | Objective: To explore the effects of DMSO-soluble fine particulate matter (PM2.5) on the contractile function in isolated rat bronchial segments and reveal its mechanism. Methods: The rat bronchi were dissected and cut into about 1 mm long segments. The bronchi were cultured in the different dose of PM2.5 and for different times. The contractile responses to carbachol (M receptor agonist) were recorded using a sensitive myograph system, and the consponding contractile time and dose effect curves were established. Results: Carbachol induced an dose-dependent contraction in rat bronchial segments. Compared to fresh, after organ culture with DMSO for 12 h or 24 h, carbachol induced the maximal contraction (Emax) and the negative logarithm of the concentration that produces 50% of the maximal contractile effect (pEC50) had not significant difference. However, after organ cultured with DMSO for 48 h, the contractile dose curves of carbachol induced contraction response shifted left significantly (P<0.01), Compared to DMSO, culture with PM2.5 for 12, 24 or 48 h, the contraction response were enhanced by carbachol in rat bronchial segments, and it was the largest for 24 h culture. Moreover, when bronchial segments were cultured with 0.3, 1.0 or 3.0 mg/L PM2.5 for 24 h，the contraction response were enhanced by carbachol, and the corresponding Emax were （149.4±9.9）%, （195.4±10.0）% and （143.9%±7.7）%, respectively. Conclusion: PM2.5 increased the M receptor-mediated contractile responses in rat bronchi and induced airway hyperresponsiveness. |
| Key words： | pharmacology; airway hyperresponsiveness; wire myograph; fine particulate matter |
| 发表期数： | 2014年12月第23期 |
| 引用格式： | 王荣，肖雪，曹蕾，等. 大气PM2.5诱发支气管的高反应性[J]. 中国科技论文在线精品论文，2014，7（23）：2367-2372. |
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