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黄芩素对大鼠心肌缺血再灌注损伤心肌酶的影响

发表时间:2014-12-15  浏览量:1295  下载量:526
全部作者: 黄勇攀,叶兰,王恒,石刚刚
作者单位: 贵阳医学院基础医学院;中南大学药学院;汕头大学医学院
摘 要: 目的:研究黄芩素对大鼠心肌缺血再灌注损伤保护作用的影响。方法:大鼠冠状动脉左前降支结扎30 min后恢复血液灌注30 min,于缺血前舌下静脉注射不同剂量黄芩素(40,80,160 mg/kg)。测定血清肌酸激酶(creatine kinase,CK)、CK同工酶(CK-MB)、乳酸脱氢酶(lactate dehydrogenase,LDH)、α-羟丁酸脱氢酶(α hydroxybutyrate dehydrogenase,HBDH)、谷草转氨酶(glutamic-oxalacetic transaminase,GOT)与超氧化物歧化酶(superoxide dismutase,SOD)的活性,以及丙二醛(malondialdehyde,MDA)的含量,并观察心肌病理形态学改变。结果:黄芩素能降低由于缺血再灌注引起的心肌损害中心肌酶CK,CK-MB,LDH,HBDH,GOT的释放,保护SOD的活性,降低脂质过氧化物MDA的产生,并呈剂量效应关系。结论:黄芩素对大鼠心肌缺血再灌注损伤具有保护作用。
关 键 词: 药理学;黄芩素;心肌缺血再灌注损伤;肌酸激酶同工酶MB;超氧化物歧化酶;丙二醛
Title: Effects of baicalein on the enzymes of myocardial tissue of myocardial ischemia-reperfusion injury in rats
Author: HUANG Yongpan, YE Lan, WANG Heng, SHI Ganggang
Organization: Basic School of Medicine, Guiyang Medical University; School of Pharmaceutical Science, Central South University; Medical College, Shantou University
Abstract: Objective: To investigate the protective effects of baicalein on rat heart ischemia-reperfusion injury. Methods: Rat heart was occluded the left anterior descending coronary artery for 30 min and then was restored blood perfusion for 30 min. Different dosages (40, 80, 160 mg/kg, respectively) of baicalein were intravenously injected before heart ischemia. The activity of plasma creatine kinase (CK), creatine kinase isoenzyme B (CK-MB), lactate dehydrogenase (LDH), α-hydroxybutyrate dehydrogenase (HBDH), grutamic oxalacetic transaminase (GOT) and superoxide dismutase (SOD), and the content of malondiadehyde (MDA) were measured. The pathologic changes were also observed. Results: Baicalein could reduce the release of CK, CK-MB, LDH, HBDH and GOT of cardiac damage, and protect the activity of SOD, while decreased the MDA product and the decrease showed a dose-effect relation. Conclusion: Baicalein may exert an apparent protective role against rat heart ischemia-reperfusion injury.
Key words: pharmacology; baicalein; myocardial reperfusion injury; creatine kinase isoenzyme MB; superoxide dismutase; malondialdehyde
发表期数: 2014年12月第23期
引用格式: 黄勇攀,叶兰,王恒,等. 黄芩素对大鼠心肌缺血再灌注损伤心肌酶的影响[J]. 中国科技论文在线精品论文,2014,7(23):2373-2378.
 
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