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rhG-CSF在C57BL/6小鼠肠型急性放射病中的作用研究

发表时间:2014-12-15  浏览量:1344  下载量:668
全部作者: 刘伟,齐丹丹,顾永平,崔凤梅,陈秋
作者单位: 苏州大学医学部放射医学与防护学院;苏州大学医学部实验中心
摘 要: 目的:通过建立急性辐射损伤动物模型,研究重组人粒细胞集落刺激因子(recombinant human granulocyte colony stimulating factor,rhG-CSF)在C57BL/6小鼠肠型急性放射病(acute radiation sickness,ARS)中的作用。方法:将健康C57BL/6小鼠随机分成5组,分别为正常组、10 Gy单纯照射组及10 Gy照后rhG-CSF高、中、低浓度给药组。照后观察记录小鼠的生存情况,绘制生存曲线;照后3.5 d取骨组织、肠组织观察形态学改变,并对肠组织进行Cleaved Caspase-3阳性细胞计数。结果:10 Gy全身照射后12 h给予高浓度rhG-CSF,小鼠生存率显著提高;照后3.5 d,rhG-CSF高浓度给药组与10 Gy单纯照射组比较,骨组织和肠组织绒毛长度变化不显著,但隐窝凋亡细胞明显减少。结论:rhG-CSF可以减少小肠隐窝部位凋亡细胞,在C57BL/6小鼠肠型急性放射病中有一定的保护作用。
关 键 词: 放射医学;重组人粒细胞集落刺激因子;肠型急性放射病;辐射防护
Title: Study on effect of rhG-CSF in intestinal form of acute radiation sickness in C57BL/6 mice
Author: LIU Wei, QI Dandan, GU Yongping, CUI Fengmei, CHEN Qiu
Organization: School of Radiation Medicine and Protection, Medical College of Soochow University; Experimental Centre, Medical College of Soochow University
Abstract: Objective: To investigate effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) in intestinal form of acute radiation sickness (ARS) in C57BL/6 mice by the animal model of acute radiation damage. Methods: Healthy C57BL/6 mice were randomly divided into 5 groupes, namely the normal grope, 10 Gy radiation group, rhG-CSF administration group of high, middle, low concentration after 10 Gy radiation. The survival curves of mice were drew after irradiation. 3.5 d after radiation, bone and intestine were taken to observe the pathological change and the number of Cleaved Caspase-3 positive cells were counted. Results: The results showed that 12 h after 10 Gy total body irradiation giving the high concentration of rhG-CSF, the survival rate increased significantly. However, 3.5 d after irradiation, comparing high concentrations of rhG-CSF administration group with 10 Gy radiation alone group, bone tissue and the length of intestinal villus did not change significantly, but the number of Cleaved Caspase-3 positive cells in crypts reduced. Conclusion: The research revealed that rhG-CSF reduced the number of Cleaved Caspase-3 positive cells in crypts and had a protective role in the intestinal form of acute radiation sickness in C57BL/6 mice.
Key words: radiation medicine; recombinant human granulocyte colony stimulating factor; intestinal form of acute radiation sickness; radio protection
发表期数: 2014年12月第23期
引用格式: 刘伟,齐丹丹,顾永平,等. rhG-CSF在C57BL/6小鼠肠型急性放射病中的作用研究[J]. 中国科技论文在线精品论文,2014,7(23):2383-2388.
 
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