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rhG-CSF在C57BL/6小鼠肠型急性放射病中的作用研究
发表时间：2014-12-15 浏览量：1677 下载量：722
| 全部作者： | 刘伟，齐丹丹，顾永平，崔凤梅，陈秋 |
| 作者单位： | 苏州大学医学部放射医学与防护学院；苏州大学医学部实验中心 |
| 摘 要： | 目的：通过建立急性辐射损伤动物模型，研究重组人粒细胞集落刺激因子（recombinant human granulocyte colony stimulating factor，rhG-CSF）在C57BL/6小鼠肠型急性放射病(acute radiation sickness，ARS)中的作用。方法：将健康C57BL/6小鼠随机分成5组，分别为正常组、10 Gy单纯照射组及10 Gy照后rhG-CSF高、中、低浓度给药组。照后观察记录小鼠的生存情况，绘制生存曲线；照后3.5 d取骨组织、肠组织观察形态学改变，并对肠组织进行Cleaved Caspase-3阳性细胞计数。结果：10 Gy全身照射后12 h给予高浓度rhG-CSF，小鼠生存率显著提高；照后3.5 d，rhG-CSF高浓度给药组与10 Gy单纯照射组比较，骨组织和肠组织绒毛长度变化不显著，但隐窝凋亡细胞明显减少。结论：rhG-CSF可以减少小肠隐窝部位凋亡细胞，在C57BL/6小鼠肠型急性放射病中有一定的保护作用。 |
| 关 键 词： | 放射医学；重组人粒细胞集落刺激因子；肠型急性放射病；辐射防护 |
| Title： | Study on effect of rhG-CSF in intestinal form of acute radiation sickness in C57BL/6 mice |
| Author： | LIU Wei, QI Dandan, GU Yongping, CUI Fengmei, CHEN Qiu |
| Organization： | School of Radiation Medicine and Protection, Medical College of Soochow University; Experimental Centre, Medical College of Soochow University |
| Abstract： | Objective: To investigate effect of recombinant human granulocyte colony stimulating factor (rhG-CSF) in intestinal form of acute radiation sickness (ARS) in C57BL/6 mice by the animal model of acute radiation damage. Methods: Healthy C57BL/6 mice were randomly divided into 5 groupes, namely the normal grope, 10 Gy radiation group, rhG-CSF administration group of high, middle, low concentration after 10 Gy radiation. The survival curves of mice were drew after irradiation. 3.5 d after radiation, bone and intestine were taken to observe the pathological change and the number of Cleaved Caspase-3 positive cells were counted. Results: The results showed that 12 h after 10 Gy total body irradiation giving the high concentration of rhG-CSF, the survival rate increased significantly. However, 3.5 d after irradiation, comparing high concentrations of rhG-CSF administration group with 10 Gy radiation alone group, bone tissue and the length of intestinal villus did not change significantly, but the number of Cleaved Caspase-3 positive cells in crypts reduced. Conclusion: The research revealed that rhG-CSF reduced the number of Cleaved Caspase-3 positive cells in crypts and had a protective role in the intestinal form of acute radiation sickness in C57BL/6 mice. |
| Key words： | radiation medicine; recombinant human granulocyte colony stimulating factor; intestinal form of acute radiation sickness; radio protection |
| 发表期数： | 2014年12月第23期 |
| 引用格式： | 刘伟，齐丹丹，顾永平，等. rhG-CSF在C57BL/6小鼠肠型急性放射病中的作用研究[J]. 中国科技论文在线精品论文，2014，7（23）：2383-2388. |
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