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神经母细胞瘤相关长链非编码RNA基因功能鉴定

发表时间:2014-12-15  浏览量:1433  下载量:453
全部作者: 于萌,朱育焱,董婉维,杨葳,郭晓冲,郑志红
作者单位: 中国医科大学实验动物部;中国医科大学附属第一医院泌尿外科
摘 要: 目的:对已在神经母细胞瘤(neuroblastoma,NB)临床标本筛选的与恶性度相关的基因——ncRAN进行其基因性质鉴定及功能学研究。方法:利用Northern blotting研究ncRAN基因是否存在表达产物,通过同位素放射自显影技术研究该基因是否在蛋白水平存在表达。利用实时PCR(real time-PCR,RT-PCR),siRNA,软琼脂克隆形成等相关实验鉴定ncRAN的生物学特性。结果:实验证明ncRAN在RNA水平上能够表达基因全长,但其在蛋白水平上不编码任何蛋白产物。通过一系列分子生物学实验鉴定,ncRAN是具有癌基因特性的基因。结论:与NB高恶性相关的,ncRAN为具有癌基因特性的长链非编码RNA(long non-coding RNA,lncRNA).
关 键 词: 基础医学其他学科;神经母细胞瘤;ncRAN;长链非编码RNA;癌基因
Title: Characteristic identification of a long non-coding RNA in aggressive neuroblastoma
Author: YU Meng, ZHU Yuyan, DONG Wanwei, YANG Wei, GUO Xiaochong, ZHENG Zhihong
Organization: Department of Laboratory Animal, China Medical University; Department of Urology, The First Affiliated Hospital of China Medical University
Abstract: Objective: To identify the characters and functions of ncRAN, which was screened from clinical sample in aggressive neuroblastoma (NB). Methods: The length of ncRAN mRNA was detected by Northern blotting. The expression of ncRAN on protein level was checked by autoradiography. The characters of ncRAN were identified by real time-PCR (RT-PCR), siRNA and soft agar colony formation. Results: The data showed that ncRAN could express the full-length gene sequences at RNA level, but it did not code for any protein products at the protein levels. The molecular biological experiment results suggested that ncRAN was a novel non-coding gene with oncogenic function. Conclusion: As a novel long non-coding RNA (lncRNA), ncRAN, acted as an oncogene, was associated with progression of NB.
Key words: other subject of basic medicine; neuroblastoma; ncRAN; long non-coding RNA; oncogene
发表期数: 2014年12月第23期
引用格式: 于萌,朱育焱,董婉维,等. 神经母细胞瘤相关长链非编码RNA基因功能鉴定[J]. 中国科技论文在线精品论文,2014,7(23):2389-2393.
 
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