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神经母细胞瘤相关长链非编码RNA基因功能鉴定
发表时间：2014-12-15 浏览量：1951 下载量：534
| 全部作者： | 于萌，朱育焱，董婉维，杨葳，郭晓冲，郑志红 |
| 作者单位： | 中国医科大学实验动物部；中国医科大学附属第一医院泌尿外科 |
| 摘 要： | 目的：对已在神经母细胞瘤（neuroblastoma，NB）临床标本筛选的与恶性度相关的基因——ncRAN进行其基因性质鉴定及功能学研究。方法：利用Northern blotting研究ncRAN基因是否存在表达产物，通过同位素放射自显影技术研究该基因是否在蛋白水平存在表达。利用实时PCR(real time-PCR，RT-PCR)，siRNA，软琼脂克隆形成等相关实验鉴定ncRAN的生物学特性。结果：实验证明ncRAN在RNA水平上能够表达基因全长，但其在蛋白水平上不编码任何蛋白产物。通过一系列分子生物学实验鉴定，ncRAN是具有癌基因特性的基因。结论：与NB高恶性相关的，ncRAN为具有癌基因特性的长链非编码RNA（long non-coding RNA，lncRNA）. |
| 关 键 词： | 基础医学其他学科；神经母细胞瘤；ncRAN；长链非编码RNA；癌基因 |
| Title： | Characteristic identification of a long non-coding RNA in aggressive neuroblastoma |
| Author： | YU Meng, ZHU Yuyan, DONG Wanwei, YANG Wei, GUO Xiaochong, ZHENG Zhihong |
| Organization： | Department of Laboratory Animal, China Medical University; Department of Urology, The First Affiliated Hospital of China Medical University |
| Abstract： | Objective: To identify the characters and functions of ncRAN, which was screened from clinical sample in aggressive neuroblastoma (NB). Methods: The length of ncRAN mRNA was detected by Northern blotting. The expression of ncRAN on protein level was checked by autoradiography. The characters of ncRAN were identified by real time-PCR (RT-PCR), siRNA and soft agar colony formation. Results: The data showed that ncRAN could express the full-length gene sequences at RNA level, but it did not code for any protein products at the protein levels. The molecular biological experiment results suggested that ncRAN was a novel non-coding gene with oncogenic function. Conclusion: As a novel long non-coding RNA (lncRNA), ncRAN, acted as an oncogene, was associated with progression of NB. |
| Key words： | other subject of basic medicine; neuroblastoma; ncRAN; long non-coding RNA; oncogene |
| 发表期数： | 2014年12月第23期 |
| 引用格式： | 于萌，朱育焱，董婉维，等. 神经母细胞瘤相关长链非编码RNA基因功能鉴定[J]. 中国科技论文在线精品论文，2014，7（23）：2389-2393. |
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