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体内体外高糖对TLR4通路炎症因子的影响

发表时间:2014-12-15  浏览量:1440  下载量:583
全部作者: 谢伟东
作者单位: 清华大学深圳研究生院,健康科学与技术重点实验室
摘 要: 目的:主要调查体外高糖和体内糖尿病对小鼠单核巨噬细胞Toll样受体4(Toll-like receptor 4,TLR4)通路炎症因子的差异性表达。方法:体外高糖培养正常小鼠的腹腔单核巨噬细胞,用定量PCR(quantitative-PCR, q-PCR)方法调查TLR4通路炎症因子的表达。体内制作链脲佐菌素(streptozotocin,STZ)的糖尿病小鼠模型,抽取腹腔单核巨噬细胞总RNA,用q-PCR方法调查TLR4通路炎症因子的表达。结果:体外高糖培养腹腔单核巨噬细胞,关于TLR4炎症通路,只有IL1a,IL1b等mRNA出现显著上调,而大多数其他因子却无明显影响。在糖尿病小鼠体内,绝大部分TLR4炎症通路上的炎症因子均出现显著上调,但这种上调相对于脂多糖(lipopolysaccharide,LPS)的作用显得十分轻微。结论:高糖对炎症通路个别因子有轻度促进作用,但其单独作用并不引起急剧或严重的炎症反应,在糖尿病小鼠体内,高糖虽然不是引起系统性炎症的关键因子,但其可能联合其他因子一起促使体内轻度慢性炎症的发生。若减少糖尿病炎症性并发症,将来的关键策略或许不是降低血糖,而是直接改善糖尿病的炎症状态。
关 键 词: 基础医学其他学科;糖尿病;炎症;Toll样受体4
Title: Effects of high glucose in vitro and in vivo on the inflammatory factors of the TLR4 pathway
Author: XIE Weidong
Organization: Key Lab of Health Science and Technology, Graduate School at Shenzhen, Tsinghua University
Abstract: Objective: This paper aims to investigate the different expressions of inflammatory factors of the Toll-like receptor 4 (TLR4) pathway in monocyte-macrophages from mouse’s peritoneal cavity induced by high glucose in vtrio or diabetes in vivo. Methods: For in vitro trials, monocyte-macrophages from normal mouse’s peritoneal cavity were cultured by high glucose and then total RNAs were extracted for quantitative-PCR (q-PCR) of inflammatory factors from the TLR4 pathway. For in vivo trials, monocyte-macrophages from diabetic mouse’s peritoneal cavity were induced by streptozotocin (STZ) and then total RNAs were extracted by q-PCR of inflammatory factors from the TLR4 pathway. Results: In the trials in vitro, high glucose only up-regulated the expressions of IL1a and IL1b did nothing to the other inflammatory factors of the TLR4 pathway. However, diabetic mice showed higher expressions of most of the inflammatory factors of the TLR4 pathway than normal controls in the trials in vivo. In addition, these up-regulations induced by high glucose or diabetes were far less than those treated by lipopolysaccharide (LPS). Conclusion: High glucose did not cause acute and extremely inflammatory responses but led to a slight increase of inflammation in monocytes or macrophages. High glucose may be not a key factor to responsible for inflammation responses but may combine with other factors to contribute low-grade systematical inflammatory responses in vivo in the case of diabetes. Future strategy should aim to control inflammation directly instead of hyperglycemia in diabetes.
Key words: other subject of basic medicine; diabetes; inflammation; Toll-like receptor 4
发表期数: 2014年12月第23期
引用格式: 谢伟东. 体内体外高糖对TLR4通路炎症因子的影响[J]. 中国科技论文在线精品论文,2014,7(23):2394-2398.
 
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