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中肋骨条藻计数及死/活分析的荧光染色法研究

发表时间:2015-02-15  浏览量:1276  下载量:345
全部作者: 孟凡鹏,陶平
作者单位: 大连海事大学环境科学与工程学院
摘 要: 以典型赤潮藻中肋骨条藻(Skelrtonema costatum)为研究对象,采用荧光染色法计数,建立双醋酸荧光素(fluorescein diacetate,FDA)荧光染色判断中肋骨条藻细胞死/活及计数的方法。结果表明:当FDA浓度为100.0 mg/L、染色时间为10 min时,对浓度为1.0×106 cells/mL的中肋骨条藻染色率为100%;采用所建立方法对含死细胞0,25%,50%,75%,100%的中肋骨条藻细胞液进行染色计数,测定值与配比值基本吻合,拟合曲线的线性相关值R2达0.997.
关 键 词: 生物无机化学;双醋酸荧光素;中肋骨条藻;荧光染色;计数;死/活分析
Title: Study on fluorescent staining of counting Skeletonema costatum and its dead/alive assay
Author: MENG Fanpeng, TAO Ping
Organization: College of Environmental Science and Engineering, Dalian Maritime University
Abstract: In this paper, a typical red tide algas, Skelrtonema costatum, was studied. Counting by fluorescent staining, a method to determine and count the dead/alive S. costatum cells was established by using fluorescein diacetate (FDA). The results showed that the dyeing rate of S. costatum (1.0�06 cells/mL) was 100%, when the concentration of FDA was 100.0 mg/L and the dyeing time was 10 min. Using the established method by counting the algae solution of S. costatum containing 0, 25%, 50%, 75%, 100% of dead cells, we found that the measured value was basically equal to the distribution value and the R2 value of the fitting curve was 0.997.
Key words: bioinorganic chemistry; fluorescein diacetate; Skelrtonema costatum; fluoresence staining; counting; dead/live assay
发表期数: 2015年2月第3期
引用格式: 孟凡鹏,陶平 . 中肋骨条藻计数及死/活分析的荧光染色法研究 [J]. 中国科技论文在线精品论文,2015,8(3):246-250.
 
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