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僵蚕抗氧化多肽的初步分离及活性分析
发表时间：2015-02-15 浏览量：1652 下载量：409
| 全部作者： | 文水莎，王彩连，杨雪海，陆如水，李灿辉，文采灵，刘文彬 |
| 作者单位： | 四川大学化学工程学院 |
| 摘 要： | 采用酶解法制备僵蚕（Bombyx batryticatus）中抗氧化肽，通过超滤进行初步分离，分析其抗氧化活性。以1,1-二苯基-2-苦味酰基苯肼（DPPH）清除率为指标，筛选出胰蛋白酶作为最适蛋白水解酶，酶解条件为温度50℃，pH=6.9，酶量0.31 g/L，底物浓度3.0 g/L，酶解时间3.67 h，对DPPH清除率为60%. 采用超滤技术获得分子量3 ku以下的小分子肽，其DPPH清除率为96%. 僵蚕蛋白水解液对DPPH有50%清除率时，底物浓度为2 g/L，其总还原力、抑制脂质体氧化能力与对照抗坏血酸相当。高温下处理2.5 h内其抗氧化活性几乎没有变化，反复冻融6次对其抗氧化活性也几乎没有影响，但在常温下随保存天数的增加其抗氧化活性减弱。 |
| 关 键 词： | 生物化学工程；僵蚕；抗氧化肽；蛋白酶解；分离纯化 |
| Title： | Purification and activity identification of white muscardin silkworm (Bombyx batryticatus) antioxidant peptide |
| Author： | WEN Shuisha, WANG Cailian, YANG Xuehai, LU Rushui, LI Canhui, WEN Cailing, LIU Wenbin |
| Organization： | College of Chemical Engineering, Sichuan University |
| Abstract： | In our current study, the antioxidant peptide was produced from enzymatic hydrolysate of white muscardin silkworm and was preliminary separated through the ultrafiltration. The antioxidant activity was analyzed. 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical-scavenging activity was used as the criterion for antioxidant activity. Peptides derived from trypsin hydrolysate exhibited the highest DPPH radical scavenging activity. Protein was enzymatically hydrolyzed at 50℃, pH=6.9, 0.31 g/L for enzyme concentration, 3.0 g/L for substrate concentration, 3.67 h for enzymatic hydrolysis time. DPPH clearance was 60%. After ultrafiltration to obtain the low-molecular weight peptide less than 3 ku, the DPPH scavenging activity arrived at 96%. The concentration for DPPH scavenging activity of 50% was 2 g/L, the total reductive activity and liposome oxidation inhibition was quite equal with ascorbic acid. High temperature treatment (boiling water bath) for 2.5 h as well as repeated freeze-thaw for 6 times, seemed not to affect the antioxi-dant activity. But the antioxidant activity decreased with the increasing storage days at room temperature. |
| Key words： | biochemical engineering; Bombyx batryticatus; antioxidant peptide; proteolysis; separation and puriffica-tion |
| 发表期数： | 2015年2月第3期 |
| 引用格式： | 文水莎，王彩连，杨雪海，等. 僵蚕抗氧化多肽的初步分离及活性分析[J]. 中国科技论文在线精品论文，2015，8（3）：290-295. |
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