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水性可降解聚氨酯PEGU25装载Bmap-18、环丙沙星或季铵盐生物缓释膜抑制细菌生物膜形成的体外研究

发表时间:2015-03-15  浏览量:1815  下载量:627
全部作者: 王建忠,刘钦瑜,王坤杰,李虹
作者单位: 四川大学华西医院泌尿外科;安徽医科大学第一附属医院泌尿外科
摘 要: 目的:通过研究多种环境下新型无毒可降解水性聚氨酯装载抗菌肽的抑菌能力,证明其作为新型抗菌生物材料的潜力。方法:用聚氨酯PEGU25(1 mg/cm2)分别装载1 mg的Bmap-18、环丙沙星或季铵盐,之后将载药膜与金黄色葡萄球菌(ATCC25923)、大肠杆菌(ATCC25922)和奇异变形杆菌(ATCC12453)一起置于培养基、人工尿液或体外人工膀胱模型中,共同培养 2,4,7 d. 采用活菌菌落计数、激光共聚焦显微镜下SYTO9/PI 荧光染色图像采集、扫描电镜(scanning electron microscopy,SEM)图像采集等方法测定细菌生长情况。结果:与空白对照相比,在培养基培养条件下,聚氨酯PEGU25装载抗菌肽Bmap-18、环丙沙星、季铵盐生物缓释膜在体外可有效抑制细菌生物膜的形成,同时可有效抑制钙镁磷酸盐沉着的硬壳细菌生物膜的形成;在人工模拟膀胱条件下,可有效抑制导管管腔内外钙镁磷酸盐沉着硬壳的形成,延缓甚至避免导管堵塞,其中环丙沙星表现出的抗菌效果最佳,Bmap-18与季铵盐相当。结论:水性可降解聚氨酯PEGU25装载抗菌肽Bmap-18、环丙沙星或季铵盐可有效抑制细菌生物膜的形成,具备服务于临床的巨大潜能。
关 键 词: 外科学;抗菌肽;细菌生物膜;聚氨酯;载药抗菌材料
Title: Biodegradable waterborne polyurethane PEGU25 incorporated in Bmap-18, ciprofloxacin or quaternary ammonium salt in the inhibition of the formation of bacterial biofilm in vitro
Author: WANG Jianzhong, LIU Qinyu, WANG Kunjie, LI Hong
Organization: Department of Urology, West China Hospital, Sichuan University; Department of Urology, The First Affiliated Hospital of Medical University of Anhui
Abstract: Objective: To test the inhibition effects of antibacterial peptide-coating-biodegradable waterborne polyurethane membrane on the biofilm formation in vitro and prove its proficiency as new antibacterial biological material. Methods: Bamp-18, ciprofloxacin and quaternary ammonium salt were incorporated in PEGU25 (1 mg/cm2), respectively. These membranes were cultured with Staphylococcus aureus (ATCC25923), Escherichia coli (ATCC25922) and Proteus mirabilis (ATCC12453) separately in culture medium, artificial urine or in vitro model of artificial bladder for 2, 4, 7 d. We evaluated the ability of inhibiting bacterial biofilm formation by living bacterium colony count, confocal fluorescence microscope combining Syto-9/PI fluorescence staining, and scanning electron microscopy (SEM) method. Results: Compared with the blank PEGU25 membrane, PEGU25 membrane with ciprofloxacin, quaternary ammonium salt, and Bmap-18 could effectively inhibit bacterial biofilm formation and the formation of calcium magnesium phosphate calm hard bacterial biofilm at the same time. Under the condition of in vitro model of artificial bladder, the formation of hard calcium magnesium phosphate calm inside and outside the duct lumen could be effectively suppressed and the obstruction of catheter could be delayed well. In which, PEGU25 with ciprofloxacin performed the best, and Bmap-18 had similar effect to quaternary ammonium salt. Conclusion: PEGU25 loading Bmap-18, quaternary ammonium salt and ciprofloxacin can well inhibit bacterial biofilm formation, which has the huge potential with service in clinic.
Key words: surgery; antimicrobial peptide; bacterial biofilm; polyurethane; drug-coating- polyurethane material
发表期数: 2015年3月第5期
引用格式: 王建忠,刘钦瑜,王坤杰,等. 水性可降解聚氨酯PEGU25装载Bmap-18、环丙沙星或季铵盐生物缓释膜抑制细菌生物膜形成的体外研究[J]. 中国科技论文在线精品论文,2015,8(5):425-436.
 
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