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家蚕作为农业生境污染物残留快速检测技术研究

发表时间:2015-03-15  浏览量:1625  下载量:601
全部作者: 柳新菊,蔡自峥,缪云根
作者单位: 浙江省农业科学院蚕桑所;浙江大学动物科学学院
摘 要: 分别从杭州市河道污染较严重的钱塘河及备塘河取污水,浸渍桑叶后喂家蚕(Bombyx mori L),调查家蚕的慢性中毒症状、死亡率、眠蚕体重等指标,并测定家蚕体内乙酰胆碱酯酶(acetylcholinesterase,AChE)及谷胱甘肽-S-转移酶(glutathione-S-transferase,GST)活性,评估家蚕作为农业生境污染物残留检测的技术可行性。结果表明:所取的不同来源污水对家蚕的影响表现为眠性中毒,从2龄起蚕始添食污水浸渍桑叶,直到4眠蚕,体重才明显低于对照蚕;但作为抗性指标的家蚕体内AChE及GST活性,却在3眠时期就已经显著降低。因此相对于表型,家蚕体内AChE及GST活性可作为农业生境污染物的前置检测指标。
关 键 词: 养蚕学;家蚕;农业生境污染物;乙酰胆碱酯酶;谷胱甘肽-S-转移酶;生物标志物
Title: Fast detection technology research of silkworm as biological indicator of agricultural contaminant
Author: LIU Xinju, CAI Zizheng, MIAO Yungen
Organization: Institute of Sericulture, Zhejiang Academy of Agricultural Sciences; College of Animal Sciences, Zhejiang University
Abstract: We took the serious polluted water from Hangzhou Qiantang river and Beitang river. The mulberry leaves were dipped in polluted water, and fed the silkworm Bombyx mori L. The chronic symptoms, mortality, body weight of sleeping worms were investigated. Also we determined the acetylcholinesterase (AChE) and glutathione-S-transferase (GST) activity to assess the technical feasibility of the silkworm as biological indicator of agricultural contaminant detection. Experimental results showed that the effects of different sources of polluted water performed on silkworm poisoning. Although starting treatment from the 2nd instar, but until the 4th sleeping stage, the body weight of worms fed with mulberry dipped in the polluted water was lower than that of control. However the AChE and GST, which was as resistance index, had been significantly reduced in the three sleeping period. Therefore, with respect to the phenotype, silkworm AChE and GST activity can be used as indicators for pre-detecting the contaminants in agricultural habitats.
Key words: sericulture science; Bombyx mori L; agricultural contaminant; acetylcholinesterase; glutathione-S-transferase; biological indicator
发表期数: 2015年3月第5期
引用格式: 柳新菊,蔡自峥,缪云根. 家蚕作为农业生境污染物残留快速检测技术研究[J]. 中国科技论文在线精品论文,2015,8(5):495-499.
 
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