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拟南芥miR858对干旱胁迫响应的研究
发表时间：2015-04-15 浏览量：1984 下载量：576
| 全部作者： | 李博文，张永力，高帅，冯圣军，杨志敏 |
| 作者单位： | 南京农业大学生命科学学院 |
| 摘 要： | miRNA（microRNA）参与并调控植物生长、发育及非生物胁迫，包括盐（NaCl）胁迫、干旱胁迫等。利用RT-PCR方法，分析200 μmol/L 脱落酸（abscisic acid，ABA）、250 mmol/L NaCl干旱（脱水）处理拟南芥（Arabidopsis thaliana）3周龄苗后，miR858 初级转录本（pri-miR858）和miR858 成熟片段（miR858）的表达水平。结果表明：pri-miR858和miR858在ABA处理2 h后被诱导表达，pri-miR858于8 h达到峰值，16 h表达量下降；而miR858在8 h和16 h表达量逐渐上升。pri-miR858和miR858 在NaCl胁迫处理2 h后被诱导表达，在8 h和16 h表达量逐渐增高。pri-miR858和miR858 在干旱胁迫处理6 h后被诱导表达到峰值，12 h表达量下降。以上分析表明：miR858可以被ABA、盐、干旱胁迫诱导表达。研究构建了35S::miR858a载体，并转化拟南芥得到转基因株系，发现35S::miR858a植株更加耐旱。 |
| 关 键 词： | 植物学；miR858；非生物胁迫；脱落酸；干旱 |
| Title： | Study on drought stress response of Arabidopsis thaliana miR858 |
| Author： | LI Bowen, ZHANG Yongli, GAO Shuai, FENG Shengjun, YANG Zhimin |
| Organization： | College of Life Sciences, Nanjing Agricultural University |
| Abstract： | miRNA (microRNA) plays important role in plant growth, development and responses to abiotic stresses including salt and drought. In this research, the expression of primary miR858 (pri-miR858) and mature miR858 (miR858) was analyzed using RT-PCR with 3-week-old Arabidopsis thaliana under the stresses of 200 μmol/L abscisic acid (ABA), 250 mmol/L NaCl and drought. Our analysis shows that the expression of pri-miR858 and miR858 are induced 2 h after treatment with ABA. Expression of pri-miR858 peaks at 8 h, and decreass at 16 h, while miR858 getting higher and higher at 8 h and 16 h. Both pri-miR858 and miR858 are induced 2 h after NaCl treatment and getting higher and higher at 8 h and 16 h. pri-miR858 and miR858 are induced 6 h after drought stress and decrease at 12 h. These results indicate that miR858 can be induced by ABA, NaCl and drought stresses. Moreover, miR858a over-expression vector is constructed and transformed to A. thaliana. Transgenic lines are obtained. We find that miR858 over-expression transgenic lines show drought tolerance. |
| Key words： | botany; miR858; abiotic stresses; abscisic acid; drought |
| 发表期数： | 2015年4月第7期 |
| 引用格式： | 李博文，张永力，高帅，等. 拟南芥miR858对干旱胁迫响应的研究[J]. 中国科技论文在线精品论文，2015，8（7）：655-663. |
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